最新刊期
      期 6 ， 2005
      • FENG Bo,ZENG Hongyan,TANG Yufang,WANG Lu
        Vol. 23, Issue 6, Pages: 321-324(2005)
        摘要：In order to explore the effect of 60Coγ−ray irradiation on minor polypeptides absorption of polystyrene microplate, an indirect ELISA detection of anti−HCV was established, 60Co γ−ray irradiated polystyrene microplates and the controls (without irradiation or UV−irradiated) were applied to absorb recombinant HCV antigens respectively. Cooperated with Bovine antihuman IgG labed HRP, their related indices of sensitivity, specificity, homogeneity and stability were determinated. The results indicated that, optimum dose of the γ−ray irradiation is 8kGy, and compared with the controls, detection sensitivity and homogeneity of the polystyrene microplate irradiated to 8kGy could be improved markedly.  
        关键词：ELISA;60Coγ−ray;Minor polypeptides;Recombinant HCV antigens   
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        发布时间：2023-03-30
      • HE Tao,JIANG Pingkai,JIA Shaojin,WANG Zhongguang
        Vol. 23, Issue 6, Pages: 329-332(2005)
        摘要：A flammability study was made for the flame retardant composites EMA(POE/ Mg(OH)2/Al(OH)3) and EMAP(POE/ Mg(OH)2/Al(OH)3/P) before and after irradiation. Limiting oxygen index (LOI), mechanical property, morphology of the char formed after combustion and thermal stability were investigated. By γ−irradiation, the LOI of EMAP increased markedly, but the LOI of EMA remained almost the same. And in the same absorbed dose, the LOI of EMAP was greater than EMA. The char structure of irradiated EMAP was more compact than before irradiation. The residue char of EMAP and EMA increased after irradiation.  
        关键词：γ−irradiation;Polyolefin elastomer;POE;Al (OH)3;Mg (OH)2;Red phosphorus   
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        发布时间：2023-03-30
      • LIN Ruotai,CHENG Wei,WEN Shengli,XIONG Guangquan,YE Lixiu,CHEN Yuxia,ZHANG Jinmu,HE Jianjun,LIN Yong,ZHAN Hanping
        Vol. 23, Issue 6, Pages: 333-336(2005)
        摘要：Ready−to−eat cooked dishes, including stir−fried dishes, steamed dishes, roast meat, deep fried dishes, shrimps and seashells, and dishes of local flavor, etc were irradiated with 60Co γ-rays, and.the decontamination effects were studied. The results showed that most of the cooked dishes are suitable for irradiation. The effective dose is 4kGy to 8kGy. Index of microbe of the irradiated dishes was conformed to the National Food−Health standards, and no significant sensory changes was observed with the irradiated dishes .The quality guarantee period (0—5℃) is 60 days.  
        关键词：Cooked dishes;Irradiation sterilization;Preserve freshness   
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        发布时间：2023-03-30
      • DENG Bo,HOU Zhengchi,ZHANG Fengying,XIE Leidong,LI Jing,YANG Haijun
        Vol. 23, Issue 6, Pages: 337-341(2005)
        摘要：Acrylic acid was grafted to Polyethersulfone (PES) powders by liquid−phase simultaneous irradiation. Effects of grafting conditions, such as absorbed dose, Volume fraction of monomer, inhibitor (Cu2+) concentration, and pH of the grafting solution on degree of the grafting were investigated. Combined with gravimetric analysis, a working curve of grafting degree through FT−IR quantitative analysis was obtained. The highest grafting degree was achieved at dose of 25kGy, Volume fraction of monomer of 30% and inhibitor concentration of 0.004 mol/L. Greater degrees of the grafting could be obtained by adding increasing amount of hydrochloric acid to the system. Hydrophilicity of the grafted PES powders increased with higher degrees of the grafting.  
        关键词：Radiation grafting;Hydrophilicity;Polyethersulfone (PES);Acrylic acid;Inhibitor   
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        发布时间：2023-03-30
      • DENG Yu,FENG Jianmin,LI Lanqingzi
        Vol. 23, Issue 6, Pages: 351-354(2005)
        摘要：A microwave treatment has been developed for bleaching wheat straw pulp by sodium hypochlorit. Effective factors on pulp bleaching, such as microwave power and irradiation time, and amount of sodium hypochlorite added, were studied. Over 77% ISO of pulp brightness from 2 gram dry pulp could be achieved by adding in it 10mL sodium hypochlorite and operating the microwave oven at 352W for 2 minutes, whereas the same effect could be achieved with 40 minutes of water bath. In addition, the microwave treatment is a water-saving process in comparison to water bath treatment.  
        关键词：Microwave;Bleach;Wheat straw pulp;Sodium hypochlorite   
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        发布时间：2023-03-30
      • YUE Ling,YANG Zhanshan,YANG Shuqin,PAN Xiuhua,PAN Peng
        Vol. 23, Issue 6, Pages: 355-358(2005)
        摘要：Antimicrobial efficacy of irradiated gentimicin was compared with the control and effect of the irradiation on potency of the gentamicin was studied. Hydrogel films containing gentamicin were prepared by radiation crosslinking technique, and mechanisms of the drug release in vitro were studied. The results indicated that potency of the gentamicin irradiated to 37.5kGy did not change. The hydrogel films incorporated gentamicin before the irradiation released gentamicin to peak during 12h, while the hydrogel films incorporated gentamicin after irradiation released gentamicin to peak during 8h. The drug released slowly up to 5d. The drug delivery conformed to Higuchi kinetic equation, and mechanisms of the drug release were matrix diffusion. By comparison, the hydrogel films incorporated gentamicin before irradiation released the drug more completely.  
        关键词：Hydrogel;Radiation;Potency;Preparation;Drug delivery   
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        发布时间：2023-03-30
      • DENG Qianchun,CHEN Chunyan,DUAN Huike,WANG Lan,XIE Bijun
        Vol. 23, Issue 6, Pages: 359-365(2005)
        摘要：Water soluble ginkgo albumin extract (GAE), which was extracted for the first time from seeds of Ginkgo bilbo L in our laboratory. has good antioxidant and anti-aging activity. In this paper, protective effect of GAE on γ−rays irradiated mice was studied. The results showed that the mice irradiated to 8.5Gy was zero, whereas survival rate of the high dosage GAE group was 20 percent. Blood picture of the 8.5Gy irradiated mice suffered damages of different degrees, while blood picture index of the GAE group decreased slower and recovered faster significantly than the irradiation control group. GAE and Vitamin C could significantly enhance serum SOD activity in serum and increase DNA content in bone marrow cells, and also promote recovery of damaged immunology function of the irradiated mice. These suggest that GAE may protect mice from the radiation damages by enhancement of antioxidant activity, hemopoiesis function and immunologic function of mices.  
        关键词：Ginkgo Albumin Extract;γ−ray irradiation;Antioxidant activity;Hemopoiesis function;Immunologic function;Protect effect   
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        发布时间：2023-03-30
      • HOU Wugang,ZHAO Jie,ZHANG Yuanqiang,ZENG Lihua,GUO Guozhen
        Vol. 23, Issue 6, Pages: 366-370(2005)
        摘要：Radiation effects caused by 100kV/m and 400kV/m electromagnetic pulse (EMP) irradiations on mouse blood−testis barrier were studied by means of routine HE staining, Lanthanum traced electron microscope and injection of caudal vein with Evans Blue. The EMP irradiation of different dose rates damaged Sertoli's cell and blood−testis barrier of mouse testis in different levels. Severe injuries were observed with the 400kV/m irradiation group, with apoptosis and necrosis in a large quantity of the spermatogenic cells, shape and structural changes of the Sertoli's cells, and serious injuries to the blood−testis barrier, one day after the irradiation. The basal compartment separated from the adluminal compartment in most of the = 8 \* ROMAN VIII stage seminiferous epithelium, and a great number of apoptosis and necrosis spermatogenic cells were released into the cavities. Injuries of blood−testis barrier could be observed 21days after the 400kV/m irradiation. The injuries of 100kV/m irradiation groups were less severe than the 400kV/m groups, in which the damages to the Sertoli's cells, the seminiferous epithelium and blood−testis barrier recovered to some extent 14days after the irradiation. We conclude that EMP irradiation can damage mouse blood−testis barrier. The injuries, and the time for recovery, are related to EMP power intensity.  
        关键词：Electromagnetic pulse irradiation;Blood−testis barrier;Sertoli's cell;Evans Blue;Lanthanum;Testis   
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        发布时间：2023-03-30
      • YANG Qiang,YUAN Mingkang,LI Mingzhen,SUN Chaoming
        Vol. 23, Issue 6, Pages: 371-372(2005)
        摘要：Steel−lead−steel samples bonded by E−51 epoxy resin were irradiated at room temperatures to doses of 0—3MGy and the mechanical properties were examined. The results indicate that for the samples irradiated to 1 or 3MGy, adhesion strength of the epoxy resin kept almost the same as the control, whereas for higher dose−irradiated samples, adhesion strength of resin decreased linearly with the dose.  
        关键词：Epoxy resin;Mechanical property;γ−ray irradiation   
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        发布时间：2023-03-30
      • HUANG Rongqing,XIAO Bingkun,LUO Chuanhuan,ZHAO Yan,LIANG Xiaodong
        Vol. 23, Issue 6, Pages: 373-374(2005)
        摘要：The work was to study anti-radiation effects of a drug extracted from lyeopodium complanatum (LC), a herb in traditional Chinese medicine. Different dosages of the LC drug were given to mice at different administration time. The survival rate and indexes of body weight difference, spleen weight, leukocyte number in the blood and nucleate cell number in the thigh bone, were measured. The results showed that the mice given 1000mg/kg of the ethanol extract of LC by PO 2h before irradiation could increase their survival rate by 20% during 30d in comparison with the control (p<0.005).  
        关键词：Lyeopodium complanatium;Ethanoextractei;Mice;Antiradiation   
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        发布时间：2023-03-30

        研究论文

      • ZHANG Xueping,ZHOU Ruimin,HE Linfeng,RAO Weihong,CHEN Yongkang,XIN Lihui
        Vol. 23, Issue 6, Pages: 325-328(2005)
        摘要：Fe3O4 nano−particles have been successfully prepared by EB irradiation with two different actived agents, Polyvinyl alcohol (PVA) and Polyethylene glycol (PEG), at room temperature and ambient pressure, without any kind of catalysts. The optical properties, component and morphology of the irradiated samples were measured by Ultraviolet-visible spectrophotometer (UV-vis), X−ray diffraction (XRD) and Transmission electron microscopy (TEM). The size of nano particles was found to be affected by the action of the different actived agents.  
        关键词：Fe3O4 nano−particles;EB irradiation;Polyvinyl alcohol (PVA);Polyethylene glycol (PEG)   
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        发布时间：2023-03-30
      • YANG Surong,LU Yingqing,GONG Qinyan,JIN Yizun,YAO Minghui
        Vol. 23, Issue 6, Pages: 342-350(2005)
        摘要：To investigate the molecular mechanisms of the anti−apoptosis effects of hematopoietic growth factors GM−CSF/IL−3 fusion protein, GM−CSF and IL−3 in Tf−1 cells induced by γ−irradiation. Caspase−3 activity was detected using fluorescence chromatometry. RT−PCR semiquantitative analysis was used to identify the expression changes of Bcl−2 and Caspase−3 mRNA, and an immunohistochemical technique was used to examine the protein expression in irradiated Tf−1 cells. The Caspase−3 activity in irradiated Tf−1 cells was markedly reduced to 15.71%—43.65% in GM−CSF/IL−3 fusion protein, GM−CSF, IL−3, and the combination of GM−CSF and IL−3−treated groups compared to those in the control groups without any growth factor. Bcl−2 mRNA and its protein expression were significantly increased by the above cytokines after incubation with the cells for 48h after irradiation, while the enhancement of the Bcl−2 mRNA level by GM−CSF/IL−3 fusion protein (10μg/L) began from 24h. After 48h of incubation after irradiation, the expression of Caspase−3 mRNA was dramatically augmented by GM−CSF/IL−3 fusion protein at 10μg/L, 100μg/L and IL−3 10μg/L, as was the corresponding Caspase−3 protein expression by the addition of each cytokine. The cytokines exerted the anti−apoptosis effects in irradiated Tf−1 cells through the up−regulation of Bcl−2 and Caspase−3 expression, and the inhibition of Caspase−3 activity.  
        关键词：Granulocyte−macrophage colony stimulating factor;Interleukin−3;γ−ray irradiation;Apoptosis   
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        发布时间：2023-03-30
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