摘要:Radiation induced graft copolymerization of functional monomers onto polymeric membrane is an important method for battery separator preparation. In order to satisfy technical requirements of different batteries, the separators should be endowed with unique electrochemical and physiochemical properties, and this can be accomplished by grafting different monomers onto polymer membranes with ionizing radiations. The paper gives a review on recent progresses in this field of research and applications.
摘要:Radiation-induced graft copolymerization is an important technologies for surface modification of polymers. In this paper, we give a review on recent progresses in this field of research. Factors affecting degree of the grafting, such as the monomer and base polymer, the solvent, the initiator and other additives, are discussed. Studies on morphologies of the grafted layers and distributions of the grafted molecular chains characterized by OM (Optical microscopy), SEM (Scanning electron microscopy), AFM (Atomic force microscopy), GPC (Gel permeation chromatography), XPS (X-ray photoelectron spectroscopy), Raman and IR (Infra-red) spectroscopy are reviewed. The emerging grafting method of living polymerization induced by ionizing radiations, with which one is able to accurately control molecular weight and structure of the grafted molecular chains, is also briefly introduced.
摘要:The study for generating and scavenging peroxynitrite anions (ONOO−) is an urgent task in free radical biology and medicine. Therefore, a novel chemiluminescence system for producing and measuring ONOO− has been established. The optimal conditions for detecting ONOO− were evaluated. The method can be described as follows. A mixture solution containing 10mmol/L hydroxylamine, 0.5mol/L NaOH and 1mmol/L EDTA was stirred vigorously in aerobic conditions for about 3h to form ONOO−. Certain amount of MnO2 powder was added to the mixture solution to eliminate H2O2 generated in the reaction. Then 100μL antioxidant and 860μL 0.1mol/L carbonate buffer (pH10) were added into a test tube. By adding 40μL filtrated ONOO− solution into the tube, chemiluminescence (CL) reaction was initiated. Ten seconds after the solution was uniformly mixed, CL intensity of the reaction solution could be determined in a measurement of 10 seconds. The method was tested with tea polyphenol, ascorbic acid, rutin and baicalin, whish are known to have effects of scavenging ONOO−. The tests show that the system is sensitive, low−cost, credible and easy method for screening ONOO−.
BAO Jianwen,ZHONG Xiangyu,LI Ye,CHEN Xiangbao,LI Fengmei,WANG Ying
Vol. 24, Issue 1, Pages: 15-22(2006)
摘要:The study was aimed at understanding behaviors of epoxy resins under electron beam irradiation, Epoxy resins of different molecular structures, which include glycidyl ether resin, glycidyl ester resin, glycidyl amine resin, and alicyclic epoxy resin, were irradiated with 5MeV electron beams. The irradiated samples were investigated with their viscosity changes, weight losses and gaseous molecules generated by the irradiation. The results show that the glycidyl ester resin and the epoxy containing ester group underwent the most serious decomposition bt the irradiation, while the glycidyl amine resin was decomposed less seriously and the glycidyl ether resin was the least decomposed.
WANG Tao,CHEN Jie,HE Simin,YANG Liming,LI Zhijun,WANG Sheng,XU Jiao
Vol. 24, Issue 1, Pages: 23-27(2006)
摘要:An ion adsorbed fabric of PP−g−NIPAAm was prepared by grafting N-isopropylacryamide (NIPAAm) onto PP (polypropylene) fabric pre-irradiated by 60Co γ−rays. The ion adsorbility were studied on the Fe2+ aqueous solution with different conditions, such as temperature, pH and ion concentration etc. The results indicated that the NIPAAm grafted PP fabric possess the ability of adsorbing the Fe2+ ions at lower pH (around pH 0) and lower temperature(5℃). The adsorbed Fe2+ ions were easily desorbed at higher temperature (50℃).
摘要:Zanthoxylum remnant was synthesized into flocculent, under microwaves, which can be used in wastewater treatment. The study shows that the best way to synthesize the flocculent is as follows. The ratio of cation and Zanthoxylum remnant is 1:1 with microwaves of 280W in power for 70s, and the best flocculation parameters is 30℃ and pH 8.
FU Zhichao,CHEN Huihua,CAI Jianming,WANG Fengmei,HAN Ling,CHEN Jianying
Vol. 24, Issue 1, Pages: 31-33(2006)
摘要:The work is to investigate the role of the P44/42 mitogen-activated protein kinases in proliferation disorder of epiglottis carcinoma induced by X-ray irradiation. After obtaining the tissues from the radiation−induced epiglottis carcinoma, we observed the P44/42MAPK protein expression level and the phosphoryation level in epiglottis carcinoma by western blotting, and the mRNA level by semi-quantitative RT−PCR. The results showed that The P44/42MAPK expression of protein and mRNA was significantly higher in lepiglottis carcinoma than in normal tissue nearby the tumor. And the level of P44/42MAPK phosphoryation in epiglottis carcinoma was higher than in that of the normal tissue. The results suggest that P44/42MAPK may play a role in proliferation disorder of epiglottis carcinoma l induced by the radiation.
关键词:Signal transduction;Mitogenactivated Protein Kinases;Epiglottis carcinoma;Radiation carcinogenesis
摘要:The work was to study in vitro the effects of ionizing radiations on the expression of 1α-hydroxylase in renal tubular epithelial cells, which were exposed to 3Gy, 5Gy and10Gy by 137Cs γ-rays. By using Real-time Q-PCR technology and transmission electron microscopy (TEM), changes in the 1α-hydroxylase mRNA level and the mitochondria ultrastructure after the irradiation were compared with the control(0Gy) group. An obvious decrease in the 1α-hydroxylase mRNA expression was found after the 3Gy irradiation, and the decreasing rate increased with the irradiation dose. The TEM results also showed that with the irradiated cells the mitochondria swelled obviously, the electron density decreased , and the cristae arrangement breaked and disordered.
YANG Jianshe,LI Wenjian,JING Xigang,JIN Xiaodong,WEI Wei,GUO Chuanling,GAO Qingxiang
Vol. 24, Issue 1, Pages: 39-42(2006)
摘要:An easy method was established for computing chromosome breaks in cells exposed to heavily charged particles. The cell chromosome break value by 12C+6 ions was theoretically calculated, and was tested with experimental data of chromosome breaks by using a premature chromosome condensation technique. The theoretical chromosome break value agreed well with the experimental data. The higher relative biological effectiveness of the heavy ions was clusely correlated to its physical characteristics. In addition, the chromosome break value can be predicted off line.
摘要:In order to investigate radiation effect on the expression of VEGF in murine bone marrow stromal cells, C57BL/6J mice were irradiated to 0, 2.5, 5 and 10Gy by whole body irradiation with 137Cs γ−ray. After the bone marrow stromal cells were ex-vivo cultured for 3, 6 or 10d, the mRNA and protein of VEGF in BMSCs were analyzed by RT−PCR and ELISA, respectively. Both of the two indices were up-regulated with the cultural time in the control group (without irradiation). In the 5 and 10Gy group, the expressions of VEGF mRNA in BMSCs increased significantly with the cultural time. Interestingly, in the 5 and 10−Gy group, VEGF concentration in BMSCs cultural media increased on 6d and reduced significantly on 10d after the ex−vivo culture. This is because that total cell numbers of the 10d group had been reduced significantly in comparison to the control group of the same cultural time. These results indicated the expressions of VEGF in BMSCs at mRNA and protein levels increased in each cell after 5 and 10Gy radiation. Up-regulated VEGF might benefit for hematopoietic reconstitution. The association among expression of VEGF, hematopoietic microenvironment and hematopoietic reconstitution deserve further study.
摘要:In order to study the effects of rhIL−11 on radiation gamages to bone marrow cells in mice induced by 4.0Gy neutron irradiation, 96 BALB/c male mice were treated with rhIL−11 by subcutaneous injection 3 d before or after 4.0Gy total body irradiation (TBI) with neutrons. The peripheral blood cells and bone marrow nuclear cells were counted. The proliferation and apoptosis of bone marrow cells were evaluated by AgNOR staining, flow cytometry and DNA gel electrophoresis, respectively. The expressions of IL−11Rαand gp130 were studied by immunocytochemistry and image analysis. After the irradiation, the peripheral blood cells, content of AgNOR and expressions of IL−11Rα and gp130 decreased obviously. However, the trends of WBC, PLT were suppressed in the group treated with rhIL−11 before the irradiation, and the trends of WBC, RBC, PLT were suppressed in the group treat with rhIL−11 after the irradiation. In both the treated groups, the content of AgNOR and expressions of IL−11Rα and gp130 are higher than those in control group. But there was not significant difference in apoptosis. IL−11 may play a positive role against injuries to bone marrow by promoting the reconstruction of bone marrow after the irradiation through IL−11Rα and gp130 signaling.
关键词:Neutron;Bone marrow;Prevention and treatment;IL−11 Receptor
摘要:Adventitious buds from in vitro leaf explants of two different Saintpaulia ionahta (Mauve and Indikon) cultivars were irradiated with X−rays or 9MeV electron beams, the tissue increase, buds differentiation and leaf morphology changes were studied. The results showed that the fresh weight of leaf explants of Mauve and Indikon irradiated to 40Gy with the X−rays increased by 27.3 and 26.3 times, whereas they increased by 49.7 and 27.4 times with the E-beam irradiation of the same dose. Changes of fresh weight increase of explants irradiated to less than 20 Gy by both X−rays and electron beams demonstrated a tendency of going down first and then increasing, which differed from those irradiated to higher than 20Gy. The percentage of bud formation of Mauve and Indikon irradiated to 100Gy with the electron beams were 3.7% and 11.3%, respectively, while they were 7.5% and 64.1% with the X−ray irradiation of the same dose. The percentage of plantlets with malformed leaves in Mauve irradiated with 60Gy E-beam irradiation was 22.2%, while it was 14.8% with X−ray irradiation of the same dose. For Saintpaulia ionahta Indikon with 40Gy E-beam irradiation, the percentage was 35.2%, whereas it was 5.8% for X−ray irradiation of the same dose. The results show that the effect of mutation induction by the electron beam irradiation on Saintpaulia ionahta is better than by the X−ray irradiation. An optimal mutagenic treatment is 40-60Gy irradiation with the electron beams.
摘要:The beam scan of a GJ-2 electron beam accelerator was improved with a 58% increase in the scan width. Dose distribution along the scan width and the irradiation technology were studied. The production efficiency has been improved to about 69%.
摘要:After whole head irradiation (WHI) of mice with 12C6+ ions (80MeV/u), changes of cycle distribution of spleen cells were studied, in an attempt to provide basic data for heavy ion radiotherapy and space radiation protection. The heads of the BALB/c mice were irradiated with 0, 0.5, 1, 2, 4 or 10Gy by the 12C6+ ions. The cell cycle distribution was analyzed by flow cytometry. Thirty-six hours after the irradiation, the S phase cells were reduced significantly (p<0.05) with increasing doses. The 0.5Gy, 4Gy and 10Gy WHI groups showed evident arrest in G1/G0 phase (p<0.05), but the 1Gy and 2Gy WHI groups had no significant arrest (p>0.05). G2/M phase of the 0.5Gy WHI group was reduced markedly (p<0.01), while the other groups showed obvious arrest (p<0.05). After WHI of mice with the heavy ions, spleen cell cycle distribution of mice changed significantly.
关键词:Neuro−endocrine System;Heavy ion irradiation;Spleen;Cell cycle distribution