最新刊期
      期 5 ， 2005
      • CAO Renping,YOU Gexin,HANG Yiping,XIAO Shimin
        Vol. 23, Issue 5, Pages: 257-261(2005)
        摘要：The paper gives a review on pre-irradiation graft co-polymerization of PP. Details are given on the method’s features, reaction mechanisms, applications and issues of radiation induced degradation of the PP membranes. PP materials grafted with functional monomers have improved properties in dying ability, felt, resistance to electrostatic charge, or hydrophile behavior.  
        关键词：Polypropylene;Grafting;Pre-irradiated;Study   
        2
        |
        0
        |
        6
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35827602 false
        发布时间：2023-03-30
      • DAI Jinming,LIN Ruxian,WANG Shengqi
        Vol. 23, Issue 5, Pages: 262-265(2005)
        摘要：Multiple pathways are involved in maintaining genetic integrity of cell after exposure to ionizing radiations. Among them, cell cycle modulation is the most important factor of radiosensitivity. DNA-strand breaks induced by the radiations can ultimately activate either temporary checkpoint that permit time for DNA damage repair or irreversible cell cycle arrest that results in cell death. The proper cell cycle arrest can offer time for DNA repair, and the modulated mechanisms therefore may be of help for cell survival in response to ionizing radiations. Further investigations in these fields will inevitably offer a new strategy for developing radiosensitive and radioprotective drugs.  
        关键词：Ionizing radiations;Cell cycle;Radiosensitivity;Radiation resistance   
        2
        |
        0
        |
        7
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35827605 false
        发布时间：2023-03-30
      • WANG Gengchao,WANG Lin,LI Xingwei,LI Chunzhong
        Vol. 23, Issue 5, Pages: 266-269(2005)
        摘要：A nanocomposite consisting of vanadium pentoxide (V2O5) and polyaniline (PAn) was prepared by in-situ intercalative polymerization, and irradiation effects on structure and properties of the PAn/V2O5 nanocomposite have also been investigated by using thermogravimetric analysis, FT-IR, X-ray diffraction, conductivity measurement and cyclic voltammetry. The results show that irradiation is of advantage to improvement of thermal stability and specific capacity of PAn/V2O5 nanocomposite for rechargeable lithium batteries. However, irradiation has no effect on interlayer spacing of V2O5 xerogel and crystalline size obviously.  
        关键词：Polyaniline;Vanadium pentoxide;Nanocomposite;60Co γ-ray irradiation   
        2
        |
        0
        |
        2
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35827670 false
        发布时间：2023-03-30
      • FENG Jianmin,DENG Yu,LI Lanqingzi
        Vol. 23, Issue 5, Pages: 270-273(2005)
        摘要：A microwave treatments has been developed for decoloration of wheat straw pulp wastewater. Granular activated carbon (GAC) and steel slag are used as catalyst. Effective factors on chrominance removal, such as quantity and ratio of the mixed catalyst, microwave power, and reaction time, were studied. Over 95% of chrominance removal from 50mL wastewater could be achieved by putting in it 10 gram of GAC and steel slag at a ratio of 1:23 and operating the microwave oven at 800W for 10 minutes. It was find that when the catalyst was used for the fourth time, the chrominance removal could still be more than 80%. A kinetics study on the treatment suggested that the decoloration process accorded to a fist-order reaction.  
        关键词：Microwave;Paper mill wastewater;Activated carbon;Steel slag;Chrominance   
        1
        |
        0
        |
        4
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35827601 false
        发布时间：2023-03-30
      • YANG Liming,SHI Lili,CHEN Jie,WANG Sheng,ZHANG Zhende,XIA Yiben
        Vol. 23, Issue 5, Pages: 274-277(2005)
        摘要：Chitosan/poly(acrylic acid) (CS/PAAc) copolymer hydrogel was prepared by E-beam irradiation of mixed aqueous solution of chitosan and acrylic acid. The effect of pH, composition and irradiation dose on the equilibrated swelling ratio of the hydrogels was determined. Experimental results show that the hydrogels swelled well in both lower pH (pH≈1) and higher pH (pH>6), however, they shrunk in the range of pH 2—5.  
        关键词：Chitosan;Poly(acrylic acid);Irradiation;Hydrogels;pH-sensitivity   
        1
        |
        0
        |
        2
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35827606 false
        发布时间：2023-03-30
      • CHEN Xiaoming,REN Zhenglong,ZHANG Huaiyu,TANG Zongxiang,LUO Peigao
        Vol. 23, Issue 5, Pages: 278-281(2005)
        摘要：A group of cultured normal human skin-derived fibroblasts was used as the cell model to investigate protective and repair effects of aqueous extracts of Mengshan green tea and Hawk tea against 320—400nm UV-ray irradiation, with the methods of MTT colorimetry and LDH release. It was found that the aqueous extracts had strong protective effect on fibroblasts against the UV-rays with dose dependence. There were no significant differences between the two kinds of tea aqueous extracts in a higher concentration of 5mg/mL, whereas at lower concentrations of 2.5 and 1.25mg/mL the, green tea aqueous extract was less effective than the hawk-tea aqueous extract in protecting fibroblasts from the UV-ray damage. Meanwhile, it was discovered that the green tea and hawk-tea aqueous extract could repair damages induced by the UV irradiation with dose dependence. But there were no statistically significant differences between the two kinds of aqueous extract. The effects may be related to antioxidant effect of tea polyphenol.  
        关键词：Green tea;Hawk-tea;Ultraviolet radiation A;Fibroblast;Damage and repair   
        1
        |
        0
        |
        2
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35827678 false
        发布时间：2023-03-30
      • ZHANG Xiaolan,BAO Borong,YE Guoan,ZHANG Xianye
        Vol. 23, Issue 5, Pages: 282-286(2005)
        摘要：For studying radiolytic products of N,N,N',N'-tetrabutyl-3-oxa-pentanediamide (TBOPDA), gas chromatography /mass spectrometry (GC/MS) was used, combined with head-space solid-phase micro-extraction (HS-SPME). The chromatograms were compared with different SPME fibres, and the results were nearly the same. Influence of the extraction temperature was also studied. The mass spectra were compared with standard spectra and analyzed artificially. Radiolytical products of TBOPDA were confirmed as Dibutylamine (DBA), Tributylamine(TBA),N,N-dibutylformamide(DBFA), N,N-dibutylacetamide(DBAA), HOCH2CON(C4H9)2, and CH3OCH2CON (C4H9)2, with HOCOCH2OCH2CON(C4H9)2 or HCOCH2OCH2CON(C4H9)2 being possible radialytic product. Comparisons with standard substances were also carried out on DBA, TBA, DBFA, DBAA. According to the radiolytic products, possible ways of the degradation were proposed.  
        关键词：Radiolytic products;N,N,N’,N’-tertbutyl-3-oxa-pentanediamide;Head space Solid-phase Microextraction;Gas Chromatography/Mass Spectrometry   
        2
        |
        0
        |
        1
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35827681 false
        发布时间：2023-03-30
      • ZHONG Zhijing,LONG Suqun,FU Yibei,XU Yunshu
        Vol. 23, Issue 5, Pages: 287-291(2005)
        摘要：Anhydrous Ethylenediamine (AEDA) was irradiated in air or nitrogen at ambient tempertures by 60Co γ-rays. The radiation degradation products were analyzed by means of GC, GC/MS, MS and FT-IR, etc. Notable gaseous products such as hydrogen, methane, ethylene, ammonia, and some volatile liquid state radiolysis products as well, were examined, and many kinds of polymerized products were detected in the liquid remain sample. It was found that under certain conditions both the hydrogen and methane contents of the products increased with dose. Remarkable effects of radiation degradation and radiation polymerization were observed, and even severe effects were found in nitrogen ambience. Therefore, a worse γ-irradiation stability of anhydrous ethylenediamine was demonstrated. The radiation polymerization of anhydrous ethylenediamine was not reported before.  
        关键词：Anhydrous ethylenediamine;γ-irradiation stability;Radiation degradation;Radiation polymerization   
        2
        |
        0
        |
        0
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35827680 false
        发布时间：2023-03-30
      • ZHANG Xiaolan,BAO Borong,YE Guoan,ZHANG Xianye
        Vol. 23, Issue 5, Pages: 292-295(2005)
        摘要：The partial electron of atoms in N,N,N',N'-tetrabutyl-3-oxa-pentanediamide (TBOPDA) was calculated with MM+ codes. The stability and cleavability of different single-bonds were discussed, with the radiation stability being C-O < C-< C-N < C-C. The calculation results were confirmed experimentally by G values of radiolysis products of TBOPDA samples, which were measured by solid phase micro-extraction and gas chromatography.  
        关键词：Radiation;N,N,N’,N’-tertbutyl-3-oxa-entanediamide;Partial electron of atom;G value   
        2
        |
        0
        |
        0
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35827732 false
        发布时间：2023-03-30
      • ZHAO Qunfen,ZHU Ying,LI Yuguo,LIU Ruili,XU Jingying,LI Qingnuan,LI Wenxin
        Vol. 23, Issue 5, Pages: 296-301(2005)
        摘要：To have a better understanding on radioprotection mechanisms of fullerenol, surviving fraction was counted for Stylonychia mytilus irradiated by 60Co γ-rays to 100—2000Gy, with fullerenol of 0.10mg mL-1 being added to some of the sample groups, on the fifth day after the irradiation. Morphologic and superstructure changes were observed with optic microscope and transmission electricity microscope. Superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), and lipofusion (LIP) levels were measured. The results showed that fullerenols could obviously enhance surviving fractions of the Stylonychia mytilus irradiated to up to 1500Gy. This indicates that the radioprotection is related to both fullerenols concentration and degree of the damages caused by the γ-ray exposure. Microscopic images revealed characteristics of the radiation damages with celluar and subcelluar structures of the Stylonychia mytilus, and these gave additional evidence for radioprotection of fullerenol. The activity of SOD and CAT of the irradiated groups are lower than the control group without irradiation (p<0.01). The presence of fullerenol obviously increased SOD and CAT activities of the irradiated group, in comparison to the control groups without fullerenol or without irradiation, with p <0.01 in both cases. The MDA and LIP levels of the fullerenol-added and irradiated groups lowered significantly than the control groups (p <0.01, p <0.05, respectively). The study shows that fullerenols have a good protection for the Stylonychia mytilus from theγ-ray exposure. Protective effect of fullerenols on damages induced by γ-rays seems to be mediated, at least in part, through their antioxidantive and radical scavenger activities.  
        关键词：Fullerenol;Rradioprotection;Stylonychia mytilus;Free radical   
        2
        |
        0
        |
        0
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35827773 false
        发布时间：2023-03-30
      • ZHU Huaping,ZHANG Yuanqiang,QI Yuhong,ZHAO Jie,ZHAO Yong,MA Jing,HOU Wugang
        Vol. 23, Issue 5, Pages: 302-306(2005)
        摘要：The work is to determine whether and where Transforming growth factor-betas downstream Signaling molecule Smad4 is expressed in the testes after whole-body X-ray irradiation and shed light on the mechanisms of Transforming growth factor-betas/Smad signal pathway mediates cell fate decisions following X-ray exposure. Five groups of adult BALB/c mice, with ten mice in each group, received whole-body of X-ray at dose levels of 0.1Gy, 0.5Gy, 1.0Gy, 1.5 Gy and 2.0Gy. They were sacrificed at 16 hour, 1 week, 2 weeks, 3 weeks and 4 weeks after the irradiation. Cellular localization and expression changes were examined by immunohistochemical ABC method. Quantitative analysis of the immunostaining was made by an image analysis system. In the seminiferous tubules, the expression of Smad4 was modulated by irradiation. The immunostaining showed that 16 hour post-irradiation, there was a significant decline in the Leydig cell, and it was dose and time depended. In addition, the immunolocalization showed that Smad4 was not exclusively localized in the cytoplasm of Leydig cells, but also localized in various Stages of spermatogenesis after the exposure, especially in premeiotic spermatogonia and primary spermatocytes. There was just a little expression in the 2.0Gy group 16h after the irradiation and the 1.0Gy and 1.5Gy groups at 2 weeks after the irradiation. Therefore in the 0.1Gy to 2.0Gy groups at 3weeks after the irradiation, the immunostaining positive cells were significantly increased in spermatogonia and primary spermatocytes. There was no significant change in sertoli cells with different doses and different times after the exposure. The different expression patterns and change by dose and time of Smad4, suggest that TGF-β/smad signal pathway may affect aspect after X-ray impairment and Smad4 may play an important role during these periods.  
        关键词：Transforming growth factor-β;Smad4;X-ray;Testicular cells;Mouse   
        2
        |
        0
        |
        2
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35827760 false
        发布时间：2023-03-30
      • GUO Wanfeng,LIN Ruxian,GUO Guozheng,WANG Shengqi
        Vol. 23, Issue 5, Pages: 307-311(2005)
        摘要：Lung cancer causes a great number of deaths worldwide, and is a leading cause of cancer death in China. Radiotherapy plays a key role in lung cancer treatments. Currently, however, it is difficult to further improve radiocurability of lung cancers. Murine double minute 2 (MDM2), an important gene in tumorigensis and cell proliferation, might be a suitable target for gene therapy approaches. In this study, we designed an antisense drug targeting to MDM2, and detected inhibitory effect of the antisense in A549 cells by RT-PCR and Western Blotting. Subsequently, the radiation-mediated cell killing was detected by Flow Cytometry and proliferative activity was observed by MTT assay. The antisense strategy was employed, too, to achieve partial reduction of MDM2 in a lung cancer cell line. This suppression of MDM2 resulted in increased radiosensitivity. The results support the hypothesis that MDM2 gene can be a candidate for radioresistance of A549 lung cancer cells, which may provide a potential molecular drug sensitizing the radioresistant cell A549.  
        关键词：Lung cancer cell A549;Radiosensitivity;Antisense oligodeoxynuclecotide;MDM2 gene   
        2
        |
        0
        |
        0
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35827758 false
        发布时间：2023-03-30
      • YAN Minfen,HAO Furong,XU Liming,TONG Shungao,JI Huajun,SHEN Zhifen,JIN Yizun
        Vol. 23, Issue 5, Pages: 312-315(2005)
        摘要：The purpose of this study is to evaluate activity changes of CYP2B1and CYP2E1 in male SD rat hepatic microsomes by γ-irradiation and/or MMC in vitro. The hepatic microsomes from blank control or phenobarital (PB)-induced rats were treated by 137Cs γ-rays (2Gy,8Gy and 16Gy), 50μmol/L MMC, or 50μmol/L MMC after the irradiation The CYP2B1 and CYP2E1 activities were determined, and were compared with the control group, which were untreated.. The results showed that the CYP2B1 and CYP2E1 activities were not changed after the irradiation in PB-induced rat liver microsomes (p>0.05); The CYP2E1 activity decreased significantly by the MMC treatment (p<0.01), whereas the CYP2B1 activity did not changed (p>0.05). There was no interaction between MMC and γ-irradiation on the CYP2B1 and CYP2E1 activities. In conclusion, the present investigations indicated that the CYP2E1 activity was inhibited significantly in 50μmol/L MMC, but not in γ-irradiation in vitro. The CYP2B1 activity was no significant changed not only under γ-irradiation but also MMC treatment in vitro.  
        关键词：γ-irradiation;Mitomycin C (MMC);CYP2B1;CYP2E1   
        2
        |
        0
        |
        1
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35827763 false
        发布时间：2023-03-30
      • SHENG Fangjun,CAO Jianping,LUO Jialin,ZHU Wei,LIU Fenju,FENG Shuang,SONG Jianyuan,LI Chong
        Vol. 23, Issue 5, Pages: 316-320(2005)
        摘要：The study is to observe effects of exogenous ATM gene on mRNA expression of hTERT (human telomerase reverse transcriptase) in fibroblast cells (AT5BIVA cells) from skin of Ataxia-telangiectasia (AT) patients and to study the regulation of ATM to hTERT. Using reverse transcription polymerase chain reaction (RT-PCR), mRNA expression of hTERT in AT、PEBS7-AT、ATM+-AT and GM cells irradiated with 0 and 3Gy of 60Co γ-rays were examined respectively. The difference of the mRNA expression of hTERT among AT、PEBS7-AT、ATM+-AT and GM cells were analyzed. Difference of the mRNA expression of hTERT between 0Gy and 3Gy groups was analyzed, too. The results showed that the mRNA expression of hTERT in GM cells was negative, but positive mRNA expression of hTERT in AT cells. The mRNA expression of hTERT in ATM+-AT cells decreased significantly (p<0.05). After exposure to 60Co γ-rays, the mRNA expression of hTERT in GM cells was positive, and that in AT、PEBS7-AT、ATM+-AT cells was increased (p<0.05). The increase of relative expression of hTERT mRNA in ATM+-AT cells was lower than that in AT and PEBS7-AT cells respectively (p<0.05). It is postulated that exogenous ATM is able to downregulate the mRNA expression of hTERT in AT cells, ionizing radiation can induce the mRNA expression of hTERT in cells and telomerase anticipates the repair of damaged DNA.  
        关键词：Ataxia-telangiectasia;ATM gene;Human telomerase reverse transcriptase;Ionizing radiation   
        1
        |
        0
        |
        0
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35827787 false
        发布时间：2023-03-30
      0