最新刊期
      期 1 ， 1993
      • Zhu Younan,Tu Tiecheng,Dong Jirong,Zhang Jiashan,Lin Nianyun
        Vol. 11, Issue 1, Pages: 1-6(1993)
        摘要：Primary radicals from the γ-radiolysis of air-saturated linoleic acid and linolenic acid at 77 K, and the subsequent secondary radicals appeared during the course of variable temperature elevation were investigated by ESR. The ESR spectrum from samples irradiated and observed at 77 K shows the presence of the radical anion doublet arised from the electron adducts of the carboxygroups and the poorly resolved broad singlet results from some carbon-centered radicals. Annealing to approximately 125 K which allow for molecular oxygen migration results in the formation of peroxyl radicals. At 247 K, the ESR spectrum is a multi-line pattern which is attributable to structure of the α-carbon radical superimposed on the pentadienyl radicals.The ESR spectra from linoleic acid——α-tocopherol and linolenic acid——α-tocopherol binary systems irradiated at 77 K and recorded at 140 K or 215 K revealed the characteristic similarity to that from α-tocopherol alone, no trace of ESR signal from either peroxyl or the composite pattern from superposition of pentadienyl radical and α-carbon radicals can be found out. Therefore α-tocopherol has exerted radioprotection effect on peroxidation of linoleic acid and linolenic acid.  
        关键词：ESR;Linoleic acid;Linolenic acid;Radical;α-tocopherol;H-atom transfer protection;Radioprotection   
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        发布时间：2023-04-23
      • Wang Zhiquan,Tang Weisheng,Chen Zhenjun,Wang Yushu
        Vol. 11, Issue 1, Pages: 7-10(1993)
        摘要：Comparative studies on frequencies of micronucleus (MN) and cells with MN from human blood lymphocytes in vitro which exposed to low dose radiation were carried out using conventional methods for culture time 50 h and 72 b and cytokinesis-block micronucleus method (CBMN). The experiment results showed that the frequencies of MN and cells with MN increased with dose. Dose-response relationship for conventional method of culture time 50 h and CBMN method were linear model, while culture time 72 h was quadratic ones; CBMN method is more sensitive and precise than conventional 2 methods; The frequencies of MN and cells with MN could be unequivocally detected if the blood was exposed to 0.1 Gy of 137Cs γ-rays in vitro.  
        关键词：Low dose radiation;Human lymphocytes;CBMN;Dose-response relationship   
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        发布时间：2023-04-23
      • Liu Jingbin,Lu Xianliang,Cao Jin
        Vol. 11, Issue 1, Pages: 11-17(1993)
        摘要：The photocrosslinking of PVC with benzophenone as photoinitiator and trimethylolpropanetrimethacrylate (TMPTMA) as crosslinking agent has been examined. Various factors affecting the crosslinking process (the concentration of photoinitiator and crosslinking agent, irradiation temperature and time, atmosphere, plasticizer) were investigated. Gel yield increased with increased irradiation time and TMPTMA content. Crosslink density increased at higher irradiation temperature and TMPTMA content. The rates of PVC crosslinking reaction were enhanced with the plasticizer.  
        关键词：Polyvinyl chloride;UV-irradiation;Photocrosslinking;Photoinitiator;Crosslinking agent   
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        发布时间：2023-04-23
      • Chen Yiqing,Ha Hongfei
        Vol. 11, Issue 1, Pages: 18-23(1993)
        摘要：In this work the radiationin-dnced deactivation of catalase in dilute aqueous solution was reported. The effects of irradiation atmosphere, temperature and original concentration of catalase, in dilute aqueous solutions on the deactivation of catalase were investigated. The protective effect by some additives (CH3CH2OH, HCOONa and EDTA) to radiation deactivation in dilute aqueous solutions was also studied. Remarkable protective effect by those additives was observed. The mechanism of radiation deactivation and protective effect have been discussed.  
        关键词：Radiation-induced deactivation;Catalase;Protective effect;Alcohol;HCOONa;EDTA   
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        发布时间：2023-04-23
      • Wu Ke,Sun Cunpu,Shi Yuanming
        Vol. 11, Issue 1, Pages: 24-27(1993)
        摘要：Electron spin resonance technique was used to investigate the ESR characteristic of watch glass irradiated with γ-ray. The result showed that signal intensity has a linear relation with irradiation dose in range of 0—50 Gy, the lower limit of detectable dose is below 2 Gy. It suggests that watch glass can be a kind of applicative material for nuclear accident dose evaluation.  
        关键词：Watch glass;ESR;γ-irradiation;Dosimetry   
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        发布时间：2023-04-23
      • Zhou Lijun,Fang Yunzhong,Zhang Yangpei,Wei Kang
        Vol. 11, Issue 1, Pages: 28-30,57(1993)
        摘要：Using the agarose gel electrophoresis in combination with laser densitometer and recording integrator, the effect of γ-ray irradiation on plasmid pBR 322 was studied. It was found that the amount of supercoiled (SC) DNA decreased with the increase of irradiation dose, whereas the nicked circular (OC) DNA increased. Formate, a well known hydroxyl free radical scavenger, could decreased the radiosensitivity of the pBR 322 DNA, indicating that the effect of the 60Co irradiation on plasmid was mainly attributable to the indirect action of radiation, Which is resulted from OH radical produced by the radiolysis of water, giving rise to single strand break of DNA.  
        关键词：γ-irradiation;DNA damage;Plasmid pBR 322;Free radical scavenger   
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        发布时间：2023-04-23
      • Wang Mingsuo,Teng Weifang,Liu Keliang,Feng Jixin,Su Liaoyuan,Xu Liying,You Guixing
        Vol. 11, Issue 1, Pages: 31-35(1993)
        摘要：The efficiency of the 60Co γ-rays irradiation induced elimination reaction of residual ethylene oxide (RETO) in the perfusion devices for single use conducted with RETO gas chromatography has been studied. The observations show: 1. the spliting reaction of RETO absorbed by the infusion sets after ethylene oxide(ETO) sterilization occured accompanied with 4kGy irradiation, therefore the relative elimination percentage (RELP) calculatted from RETO is over 90%. 2. the optimum radiation conditions are as following: irradiation dose 4 kGy, dose rate 8 Gy/min, irradiation temprature 20℃. 3. the RETO content within medical infusion sets at 2 weeks storing time after radiation was probably controlled under 2.0 ppm. 4. the radiation chemistry mechanism of the RETO elimination reaction is discussed in relation to the primary and secondary actions of elimination reaction.  
        关键词：Gas chromatography;Perfusion devices;Ethylene oxide;Radiolytic effect;Chemical sterilization   
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        发布时间：2023-04-23
      • Zhu Shengtao,Kume T
        Vol. 11, Issue 1, Pages: 36-39(1993)
        摘要：In this paper, a 5-day germination test was used to identify irradiated wheat. Germination rate decreased when radiation dose was up to 3.3 kGy. Nearly no seeds shot at 10 kGy. At 0.5 or 1.0 kGy irradiation did not decrease germination rate. It significantly inhibited the growth of shoots and roots. Shoot length was about 20 mm which could not be elongated by prolonged germination test. The effects of old seeds, post-irradiation storage and wheat varieties on germination were discussed.  
        关键词：Wheat;Irradiation;Detection;Germination   
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        发布时间：2023-04-23
      • Lin Xiao,Chen Jie,Xu Jiping
        Vol. 11, Issue 1, Pages: 40-43(1993)
        摘要：In this paper, the gas permeabilities of the membranes of copolymer of trimethylsilyl propyne with pentamethyldisilyl propyne (PTMSP-PMDSP) treated by octafluorocyclobutane (OFCB) plasma were studied. The effect of plasma deposition conditions such as deposition time and monmer pressure was studied. At a power of 250 W, OFCB pressure of 5.2 Pa, and deposition time of three minutes, oxygen permeability and oxygen-nitrogen separation factor of the composite membrane were 1020 barrer and 4.18, respectively. The surface composition of the composite membrane was characterized by X-ray photoelectron spectroscopy (XPS). XPS data of plasma modified and unmodified the membranes showed a drastic alteration in the surface structure and a significant decrease in Si/C atomic ratio in the surface layer. It Was not found any relationship between the magnitude of the F/C atomic ratio for these films and their ratio.  
        关键词：Polytrimethylsilyl propyne;Plasma modification;Octafluorocyclobutane;Oxygen;Nitrogen;Gas permenability   
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        发布时间：2023-04-23
      • Lu Zhaoxin,Takashi Fujimura
        Vol. 11, Issue 1, Pages: 44-48(1993)
        摘要：The immobilization of yeast cells was carried out by using the copolymer produced by radiation polymerization of hydroxyethyl acrylate (HEA) and glyciolyl methacrylate (GMA) monomer at - 78℃ low temperature. The immobilized cells with the copolymer, poly (HEA-GMA) had higher ethanol productivity than free cells. However, the ethanol praductivity of immobilized cells varied with the composition of copolymer, in which the ethanol productivity of immobilized yeast cells with the copolymer from 17% HEA and 6% GMA was the highest, 29 mg/ml•h, increasing by 3 times in comparison with that of free cells. And it was obvious that the activity of immobilized yeast cells was higer when the concentration of monomer was 20%—30%. In this work, the relation between the properties of copolymer and the ethanol productivity of immobilized yeast cells was also investigated.  
        关键词：Copolymer;Radiation polymerization;Immobilization;Yeast cells;Ethanol   
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        发布时间：2023-04-23
      • Wang Chongdao,Lao Qinhua,Qiang Yizhong,Shen Weiming
        Vol. 11, Issue 1, Pages: 49-52(1993)
        摘要：Lipid peroxides (LPO) in the tissues of mice was determenined by the modified spectrophotometry with TBA. The results showed: 1) LPO contents in liver, spleen, kidney and bone marrow of mice dramatically increased and their proteins markedly reduced at the 15 th day after internal radiation. There exists negative relationship between them. 2) A gradual increase of LPO contents in liver, spleen and kidney of mice at the 72 th hour was followed by an increase of whole-body gamma radiation at a dose from 2 to 4 Gy. A linear relationship between the dose and LPO contents was observed in the dose range of 0—4 Gy. 3) Tannic acid and squalene had certain anti-LPO effects.  
        关键词：γ-radiation;Lipid peroxides;Mouse;Tannic acid;Squalene;Spectrophotometry   
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        发布时间：2023-04-23
      • Zeng Guiying,Chen Binfu,Liu Aiping,Zhou Yuankai
        Vol. 11, Issue 1, Pages: 53-57(1993)
        摘要：The intestine homogenate of mice was undergone stepwise procedure of heating, centrifugation, salting out and nucleic acid isolation, and each fraction was subjected to biological activity assay using the method of crypt counting per unit intestine area in mice. The results showed that the nucleic acid extracted from intestine homogenate, either DNA or RNA, was capable of enhancing the crypt survival in mice after γ-irradiation. These are consistent with our previous results obtained by using the crude extract of intestine homogenate. Therefore, the crypt survival enhancing entities in intestine homogenate of mice are identified to be nucleic acids.  
        关键词：Biological activity assay;Efficacious component;Nucleic acid;Irradiation;Intestinal gland recovery   
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        发布时间：2023-04-23
      • Zhu Shuxiu,Wang Peizhi,Wang Yanling,Wei Yubo,Huang Quanshen,Fan Zheru
        Vol. 11, Issue 1, Pages: 58-61(1993)
        摘要：An investigation on increasing crop output and its mechanism was conducted in four replication in 1988—1989. The crops in the trial were winter wheat and wheatgrass. The irradiation dose of 60Co γ rays was 2.5×104Gy. The experimental result showed that the dry matter, N content of stalk and leaves above ground of wheatgrass and kernels yield of winter wheat had a considerable increase of 86.49%, 38.68% and 48.41% respectively after soil irradiation. The AN value of soil was from 1616.8 mg before irradiation to 5112.8 mg after irradiation, and 15N aboundance of stalk and leaves of wheatgrass in irradiated soil had decrease of 50.45% comparing with unirradiated soil. The content of nutrient elements in soil before and after irradiation was assaied. The availble N, P, K and Mn had an increase of 21.30%, 30.75%, -10.79% and 46.97% respectively after irradiation. The organic matter content, pH value and radioactivity in the soil before and after irradiation had no significant changes.  
        关键词：Soil;γ-radiation;Nitrogen content;Winter wheat;Wheatgrass   
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        发布时间：2023-04-23
      • Chen Deqing,Zhang Zhaoyang,Zhou Xiangyan
        Vol. 11, Issue 1, Pages: 62-64,F4(1993)
        摘要：Mice were abdominally injected with a adaptive dose of 3.7× 102—3.7×105 Bq/gbw HTO, and then exposed to a challenge dose of 1.5 Gy of 60Co γ-rays. In bone marrow cells that received both the adaptive and challenge doses, the chromatid breaks are lower than expected on the basis of additivity of the effects of the individual treatment. The adaptive response induced with 3.7×103Bq/gbw HTO is the most remarkable, but at 3.7×105Bq/gbw the adaptive response seems to be disappear. The adaptive response can be observed by exposing to 1.5 Gy γ-rays from 1 to 5 days after injection of 3.7×103Bq/gbw HTO, which is the most obvious at the 5 th day, to reduce chromatid breaks to 50% of expected, but at the 7 th day to equat to expected. The frequency of chromatid braeks is gradually reduced with time after challenge dose, the maximum index number of adaptive response is 0.50 and appears at 24 hr after challenge dose.  
        关键词：Adaptive;HTO;Mouse chromatid aberration;Response   
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        发布时间：2023-04-23
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