最新刊期
      期 2 ， 1999
      • LI Jun,ZHAI Maolin,YANG Pengxiang,GAO Hongcheng,HA Hongfei
        Vol. 17, Issue 2, Pages: 65-69(1999)
        摘要：In this work, the direct radiation graft polymerization of NIPAAm onto silicone rubber was performed. It was found that the grafting yield was increased with dose up to 40kGy, but decreased with dose rate from 50 to 500 Gy/min. The graft reaction could be performed in air,but it had a obvious induction period. The grafting was also enhanced by raising concentration of monomers and by acid as well. The characterization of grafted films was measured. The surface of the grafted films exhibited thermal sensitivity and the LCST of them closed to 35 ℃ (by DSC) , so the differences of swelling percent in water at different temperature (below or above LCST) was observed obviously. The surface morphology of the samples was observed by SEM.  
        关键词：Silicone rubber;NIPAAm;Direct radiation grafting;Thermo —sensitive polymer;Surface modification   
        2
        |
        0
        |
        6
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35963488 false
        发布时间：2023-04-07
      • WANG Huiliang,KONG Xiangbo,CHEN Wenxiu
        Vol. 17, Issue 2, Pages: 70-75(1999)
        摘要：The effects of hindered amine light stabilizers (HALS) , antioxidants and the combination of HALS and antioxidants on the radiation — induced discoloration of polypropylene (PP) are studied. The yellow index (YI) of PP added pentamethyl HALS is found to be lower than that of PP added corresponding tetramethyl HALS at the same radiation dose. The YI of PP added antioxidant Irganox 1076 is lower than that of PP added Irgafos 168 or Irganox PS —802. When Irganox 1076 and PDS are used together, the YI of PP is lower than that of Irganox 1076 or PDS used separately. YI of PP decreases with storage time after irradiation. The results are discussed.  
        关键词：Hindered amine light stabilizer(HALS);Radiation—induced discoloration;Antioxidant;Polypropylene   
        2
        |
        0
        |
        8
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35963484 false
        发布时间：2023-04-07
      • GUO Kai,LI Jun,YI Min
        Vol. 17, Issue 2, Pages: 76-79(1999)
        摘要：Photografting of acrylic acid (AAc) on poly (ethylene terephthalate) (PET) film surface was carried out in aqueous solution. The effects of photo sensitizer, monomer concentration and reaction temperature on the grafting yield were investigated. The grafted film exhibited high wettability.  
        关键词：Poly (ethylene terephthalate) (PET) film;Acrylic acid;Photografting   
        2
        |
        0
        |
        0
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35963481 false
        发布时间：2023-04-07
      • KONG Xiangrong,DU Zeji
        Vol. 17, Issue 2, Pages: 89-92(1999)
        摘要：In this study, the radioresistance of wild type Deinococcus radiodurans KD8301 and the factors affecting the radioresistance were investigated. KH3111 which was a DNA repair mutant of KD8301 (Zeji Du, 1998) was used to be compared with KD8301. Deinococcus radiodurans was discovered by Anderson et al (1956) in X —ray sterilized canned meat that was found to have undergone spoilage. This bacterium and other species of this genus share extreme resistance to ionozing radiation and other agents that damage DNA. Wild type KD8301 and its sensitive mutant KH3111 were irradiated with 60Co γ—ray at the dose range 0.5~10kGy. Dose — survival fraction curves were made and the radio resistances were determined by LD99. The relative contents of DNA in cells were measured by Fluorescence Spectrophotometry (Freedman and Bruce, 1971) . The results indicated that wild type KD8301 possesses more radoresistant than its mutant KH3111, LD99 were 9.5kGy and 2.4kGy respectively. KD8301 grown at exponential phase showed a decreased resistance to radiation, and the LD99 was 5. 1kGy. No differences of DNA/protein in cells were found between the exponential phase and the stationary phase. The results could be concluded that wild type KD8301 possesses remarkable radioresistance, but this ability was decreased or disappeared after mutation (in KH3111) . None DNA relative content other than the growthstages were determinant factors of radioresistance in Deinococcus radiodurans. This results were different from other report (Dickie N et al, 1990) . The cellular mechanisms might be the defferences of the bacterium cell morphology between the exponential phase and the stationary phase. Recently, the mutation site of KH3111 which was mutated chemically from wild type KD8301 was identified (Zeji Du, 1998) . One base pair changed in the novel gene pprA which was isolated from KD8301 genomic DNA. This point mutation was confirmed to be responsable for the sensitivity of KH3111 to γ—ray and other DNA—damaging agents.  
        关键词：Radioresistant bacterium;γ—ray;Radioresistance   
        2
        |
        0
        |
        1
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35963478 false
        发布时间：2023-04-07
      • LIU Jiamei,CHEN Dong,LIU Shuzheng
        Vol. 17, Issue 2, Pages: 93-96(1999)
        摘要：The relationship of time—effect and dose—effect of apoptosis in mouse Peyer s patches after whole body irradiation (WBI) with different doses of X —rays was studied by the method of TdT —mediated dUTP nick end labeling (TUNEL) . The results showed that the number of TUNEL positive cells in mouse Peyer's patches were significantly increased following WBI with 2 Gy irradiation,While the number of TUNEL positive cells were decreased after WBI with doses of 0.05Gy and 0.075Gy X —rays. The results support the view that 2Gy irradiation promote the apoptosis of immune cells and the low doses of radiationsuppress theapoptosis of immune cells.  
        关键词：Ionizing radiation;Peyer's patches;Apoptosis;TUNEL   
        2
        |
        0
        |
        2
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35963569 false
        发布时间：2023-04-07
      • LUO Can,SU Xu,LIU Jianxiang,FU Shibo,JU Guizhi
        Vol. 17, Issue 2, Pages: 97-100(1999)
        摘要：It has been demonstrated that the activation and proliferation of T lymphocytes is a crucial link in enhancing immune function by low dose ionizing radiation. Transferrin receptor (TfR) is one of the important membrane receptors expressed in T cells during activation and proliferation of T lymphocytes and is closely related with the activation and proliferation of T lymphocytes. TfR expression in lymphocytes in mouse spleens was examined using direct immunoflurescence and flow cytometric analysis. The results showed that whole body irradiation (WBI) with low dose X —rays could stimulate TfR expression in lymphocytes in mouse spleens, and this might be another evidence for enhancing immune function with low dose radiation and meanwhile indicated that TfRs play an important role in enhancing immune function with low dose radiation.  
        关键词：TfR;Splenocytes;X —rays;Low dose radiation   
        2
        |
        0
        |
        0
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35963575 false
        发布时间：2023-04-07
      • CUI Yufang,YANG Hong,GAO Yabing,XIONG Chengqi,XIA Guowei,GAO Linlu,WANG Dewen
        Vol. 17, Issue 2, Pages: 101-106(1999)
        摘要：To investigate the role of apoptosis in radiation—induced damage and repair of mouse spleen and provide the basis for expounding the corresponding mechanism as well as for the prevention and treatment of acute radiation. Using in situ terminal labeling (ISTL) , DNA electrophoresis and immunohistochemical techniques, we studied the dynamic changes of apoptosis of mouse spleen lymphocytes and the expression of bax and bcl—2 gene products after 2,4, 6 and 8Gy whole body γ—irradiation. Results (1) At the early stage after irradiati on the percentages of lymphocyte apoptosis increased rapidly, for instance, 4h and day 1 after 6Gy γ—irradiation the apoptotic lymphocyte were 4.9 and 9.7 times those of control values; At the same time, with the increase of radiation doses the percentages of lymphocyte apoptosis increased rapidly, for instance, 8h after 2, 4, 6 and 8 Gy γ—irradiation the apoptotic lymphocyte were 3.7,5.4, 6.2 and 8. 3 times those of control values respectively. (2) After 6 Gy γ—irradiation typical morphological characteristics of spleen apoptotic lymphocyte in early, middle and late stages were observed by transmission electron microscope. Using DNA gel electrophoresis technique the spleen lymphocytes displayed characteristic DNA ladders 4 and 6 h after 6 Gy γ—irradiation. The percentage of lymphocyte apoptosis measured by ISTL was 3.4 times higher than that of control value 4 h after 6 Gy γ—irradiation. (3) Abnormal expression of bcl —2 and bax gene products in irradiated lymphocytes confirmed that they played a vital role in the apoptosis of mouse spleen lymphocytes. Conclusion Apoptosis played an important role in the whole process of radiation—induced damage and repair of mouse spleen lymphocyte and Bcl—2 and Bax proteins could regulate the process of lymphocyte apoptosis.  
        关键词：Lymphocyte apoptosis;Irradiation;Bax and Bcl—2 proteins;Mouse spleen   
        2
        |
        0
        |
        8
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35963580 false
        发布时间：2023-04-07
      • ZHU Hanneng,SUN Daren,LUO Weihua,SHI Jianhui,CHENG Wenying
        Vol. 17, Issue 2, Pages: 107-110(1999)
        摘要：To investigate the radiation — induced apoptosis of human fibroblasts with different radiosensitivity, the methods of TUNEL and FCM were employed to test the apoptosis of cells treated with 60Co γ—rays. The results of experiments indicated that : (1) Surviving fraction experiment identified the No 6 fibroblast cells have a high radiosensitivity than that of No 8 cells after exposured to 60Co γ—rays; (2) Apoptosis rate has a positive relationship with radiosensitivity by the methods of TUNEL and FCM, in accordance with the result made by the surviving fraction experiment; (3) Both apoptosis rate and radiosensitivity increased within the 48 hours after radiation. In this conclusion is that : The changing of apoptosis rate can reflect the radiosensitivity level of cell line; the changing of cellular apoptosis rate and radiosensitivity were time—dependant after radiation. it is possible that apoptosis may be an important marker for judging cellular radiosensitivity.  
        关键词：Human fibroblast;Apoptosis;Radiosensitivity   
        2
        |
        0
        |
        1
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35963574 false
        发布时间：2023-04-07
      • YE Fei,LIU Shuzheng
        Vol. 17, Issue 2, Pages: 111-114(1999)
        摘要：The effect of whole body irradiation (WBI) with various doses on cell cycle progression of splenocytes in mice was investigated using flow cytometry. The results showed that DNA synthesis was stimulated within 12~72h after 75mGy irradiation, reaching the level of sham—irradiated group on day 7. Meanwhile,after WBI with 2.0Gy X —rays DNA synthesis was suppressed with significant G2 block beginning from 8h, and retruned the level of sham—irradiated group on day 7.The study of dose—effect relationship showed that DNA synthesis was enhanced 24h after 50 and 75 mGy X—rays, and G1 block occured after WBI with 100 and 200mGy, but DNA synthesis was suppressed with G2 block and cell division delay 24h after WBI with 0.5~4. 0Gy.  
        关键词：Whole body irradiation;Splenocytes;Cell cycle;G1 block;G2 block   
        2
        |
        0
        |
        6
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35963568 false
        发布时间：2023-04-07
      • YAN Minfenqquad,JIN Yizunqquad,TONG Sungao,XU Liming
        Vol. 17, Issue 2, Pages: 115-118(1999)
        摘要：The purpose of the present study was to investigate whether the sulfonamide—imidazole (IVb) had radiosensitization effects on H22 tumor. Mice with H22 epatoma were randonized into eight groups:control; radiation alone; radiation+ip 0.58g/kg IVb; radiation+ip 0.29g/kg IVb; radiation+ip 0.14g/kg IVb; ip 0.5g/kg IVb alone; ip 0.29g/kg IVb alone; ip 0.14g/kg IVb alone. IVb was diluted with normal saline and administered to the mice before irradiation. The dose of radiation is 10Gy. Each group was treated mentioned above[1]. The volume of mice's tumors was measured every two days[2]. We calculated relative tumor growth rates[3], tumor inhibition rates and days of tumor growth delay[4]. At the doses of 0.58g/kg, 0. 29g/kg and 0.14g/kg IVb with γ—ray radiation,the relative growth rates decreased and the tumor inhibition rates, the days of tumor growth delay increased, respectively. Among the three doses, the inhibition effects on tumors was most significant at the dose of 0.29g/kg. There was no effect when IVb was adimin istered alone. The results indicated that IVb has some radiosensitization effects on H22 hepatoma in vivo.  
        关键词：1—substituted—4—sulfonamide—imidazole;Radiosensitizer;H22;Tumor relative growth rate;Tumor inhibition rate;Tumor growth delay   
        2
        |
        0
        |
        3
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35963640 false
        发布时间：2023-04-07
      • ZHU Hanneng,LIU Yuanxia,FANG Yan,SHI Jianhui,CHENG Wenyin
        Vol. 17, Issue 2, Pages: 119-121(1999)
        摘要：In order to test the radiosensitivity of normal skin tissure, we cultured cancer patient's skin tissure fibroblast, Surviving fraction experiment was employed to provide data for understanding of the different radiosensitivity among the cancer patients, Medthod: cancer patient's skin tissure fibroblast were cultured in vitro by the way of tissure attachment, cells wereirradiated by graded doses of γ—ray,cell dose response experiment was used to test the radiosensitivity of cell. Result : Cancer patient's skin fibroblast could be propagated and passaged by the method of culture in vitro. Radiosensitivity are different among the various cancer patient s skin tissure fibroblasts.  
        关键词：Skin tissure fibroblast;Culture in vitro;Radiosensitivityend document   
        2
        |
        0
        |
        0
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35963644 false
        发布时间：2023-04-07
      • LIANG Yan,ZHANG Ming,LIU Shuzheng
        Vol. 17, Issue 2, Pages: 122-124(1999)
        摘要：To observe the effects of low dose radiation on cAMP levels of pineal gland. We examined the cAMP contents of pineal gland after whole—body irradiation with 50, 75, 100, 200, 500mGy X—ray on Kunming male mice. The results showed that the cAMP levels of pineal gland after WBI with 50~100mGy X—ray in mice was higher than sham irradiation mice, especially, 75mGy group was marked higher than that of sham irradiation group (p<0.001) . Whereas, high dose radiation may reduced to the cAMP levels of pineal gland in mice, among 2、4Gy groups was lower than that of sham irradiation group.  
        关键词：Low dose radiation;Pineal gland;cAMP   
        2
        |
        0
        |
        0
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35963643 false
        发布时间：2023-04-07
      • LI Xiujuan,YANG Ying,LI Xiuyi,LIU Shuzheng
        Vol. 17, Issue 2, Pages: 125-128(1999)
        摘要：To study immunologic mechanism of the suppressive effect of low dose radiation (LDR) on thymic lymphoma (TL) induced by high dose radiation (HDR) . We adopted the model that C57BL/6J mice were administered whole body irradiation with 1.75Gy X—rays one time every week for 4 weeks to induce TL. It was examined that splenic NK cytotoxic activity, IL—2 and γ—IFN secretion activity, peritoneal macrophagic phagocytosis and its TNF—α secretion activity in mice with different dose 1 month after irradiation. The results showed that all the immunologic functions mentioned above in mice given 75mGy 12h before 1.75Gy every time were higher than that in mice given only 1.75Gy, and approached to the sham —irradiation mice. It suggested that the suppressive effect of LDR on TL induced by HDR may be related to the adaptive response induced by LDR and decreasing immunological functions damage caused by HDR.  
        关键词：Low dose radiation;High dose radiation;Thymic lymphoma;Immunological functions   
        2
        |
        0
        |
        4
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35963647 false
        发布时间：2023-04-07

        研究论文

      • DU Zeji,KONG Xianrong,Issay Narumi,Hiroshi Watanabe
        Vol. 17, Issue 2, Pages: 80-88(1999)
        摘要：Deinococcus radiodurans （Dr） possesses a prominent ability to repair the DNA injury induced by various DNA — damaging agents including mitomycin C （MC）, ultraviolet light（UV） and ionizing radiation. DNA damage resistance was restored in MC sensitive （MCs） mutants 2621 and 3021 by transforming with DNAs of four cosmid clones derived from the gene library of strain KD8301 which showed the property of wild type phenotype to DNA —damaging agents. Gene affected by mutation （mtcA or mtcB） in both mutants was cloned and its nucleotide sequence was determined. The deduced amino acid（aa） sequence of Dr uvrA gene product consists of 1016 aa and shares homology with many bacterial UvrA proteins. The mutation sites in both mutants were identified by analyzing the polymerase chain reaction （PCR） fragments derived from the genomicDNA of the mutants. A 144—base pairs （bp） deletion including the start codon for the uvrA gene was observed in DNA of the mutant 3021,causing a defect in the gene. On the other hand,an insertion sequence （IS） element intervened in the uvrA gene of the mutant 2621, suggesting the insertional inactivation of the gene. The IS element comprises 1322 —bp long, flanked by 19—bp inverted terminal repeats （ITR）, and generated a 6—bp target duplication （TD）. Two open reading frames （ORF） were found in the IS element. The deduced aa sequences of large and small ORF show homology to a putative transposase found in IS4 of Escherichia coli E. coli） and to a resolvase found in IS Xc5 of Xanthomonas campestris （Xc）, respectively. This is the first discovery of IS element in deinobacteria, and the IS element was designated IS2621.  
        关键词：DNA repair;uvrA gene;Insertion sequence;Deinococcus radiodurans   
        2
        |
        0
        |
        0
        <HTML>
        <L-PDF><Meta-XML>
        <引用本文> <批量引用> 35963485 false
        发布时间：2023-04-07
      0