最新刊期
      期 2 ， 2000
      • LI Hongru,GU Jiangnan,YU Shangxian
        Vol. 18, Issue 2, Pages: 81-85(2000)
        摘要：As a hydrogen abstracting photoinitiator, sulfo-benzophenone has low volatility, high sensitivity and broad photoresponse range. The binary photoinitiating system composed of sulfo-benzophenone and tertiary amine has even higher photoinitiating efficiency. Three sulfo-benzophenone compounds and a new aliphatic tertiary amine were synthesized. The photographic sensitivities of binary photoinitiating systems composed of sulfo-benzophenone and tertiary amine synthesized were measured.  
        关键词：Hydrogen abstraction photoinitiate;Residual step;Sulfo-benzophenone;Tertiary amine   
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        发布时间：2023-04-07
      • FU Haiqing,JU Guizhi,SU Xu,FU Shibo
        Vol. 18, Issue 2, Pages: 86-90(2000)
        摘要：To elucidate the role of p53 and its down-stream gene in G1 arrest of mouse thymocytes induced by ionizing radiation, Flow cytometry (FCM) and northern blot were used to analyze the changes of cell cycle, expression of p53 and its down-stream gene of thymocytes of Kunming mice after whole body irradiation (WBI) with X-rays. The results indicated: (1) The number of G1 phase cells of thymocytes began to significantly increased at 4h after WBI with 2Gy X-rays, and reached the peak level at 24h, and kept a higher level until at least 48h. (2)The percentages of p53 + cells of mouse thymocytes increased from 1h to 8h, and p21+ from 4h to 8h after 2Gy WBI. However, no change was found in the percentage of GADD45 + cells of mouse thymocytes. (3) WAF1 mRNA level began to increase at 1h, and reached the peak level at 2h, and kept a higher level till to 24h, and recovered to sham-irradiation level at 48h after WBI. There was no change of GADD45 mRNA level, in accordance with its protein level change. These results suggested that p53-WAF1 pathway may be play an important role in the G1 arrest of mouse thymocytes induced by ionizing radiation.  
        关键词：Ionizing radiation;G1 arrest;p53-WAF1 pathway   
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        发布时间：2023-04-07
      • MA Shumei,FU Shibo,JU Guizhi
        Vol. 18, Issue 2, Pages: 104-107(2000)
        摘要：The changes of cell cycle progression in mice bone marrow hemopoietic cell and the dose-effect relationship after X-ray whole body irradiation (WBI) were studed by flow cytometry (FCM). The data showed the elevation of the percentage of cells in G1 phase 12h after irradiation (p <0.05), the decrease of cells in S phase 4h (p <0.05) and the increase of cells in G2+M phase 4h and 12h after 2.0Gy WBI. 12h after 0.5~6.0Gy irradiation the percentage of cells in G1 phase became higher than that of sham-irradiation, especialy in 1.0, 2.0, 4.0 and 6.0Gy groups (p < 0.05 ~p <0.01), both the decrease of S phase percentage (p < 0.05 ~p <0.01)and the increase of G2+M phase (p < 0.05 ~P <0.001) was in a dose-dependent manner. Our results suggested that 2.0Gy X-ray WBI to mice might induce the arrest in G1 and G2 phase and the suppression of DNA synthesis, these changes were all in dose- dependent manner within 0.5~6.0 Gy.  
        关键词：Ionizing irradiation;Cell cycle;Bone marrow hemopoietic cells   
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        发布时间：2023-04-07
      • SUN Yimin,LIU Shuzheng
        Vol. 18, Issue 2, Pages: 108-112(2000)
        摘要：We have examined the interaction among peritoneal macrophages, splenic accessory cells and splenic lymphocytes after whole body-irradiation (WBI) of mice with X-rays. The results showd: WBI with 75mGy X-rays could increase the proliferative response of splenic lymphocytes to ConA treatment, while 2 Gy WBI decreased it. If the splenic cell suspension was depleted of adherent accessory cells, proliferative response of lymphocytes was lowered. Peritoneal macrophages and splenic accessory cells obtained from mice exposed to lower dose radiation induced increase of proliferative response of T lymphocytes to ConA and IL-2 production.  
        关键词：Macrophages;Splenic accessory cells;Irradiation   
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        发布时间：2023-04-07
      • YANG Zhanshan,ZHU Nankang,YANG Shuqin,MI Zhisu
        Vol. 18, Issue 2, Pages: 113-116(2000)
        摘要：Toxicity and healing effect of poly(ethylene oxide) (PEO)/poly(vinyl alcohol) (PVA) blend hydrogel films as wound dressings were evaluated. Toxicity testing is checked by acute general toxicity, in vitro cytotoxicity and sticking on skin assay. We used a highly sensitive 7 d RPMI-1640 cell culture with fibroblasts as cytotoxicity test system. Cytotoxicity is assessed by monitoring cell growth inhibition, supported by cell morphological evaluation using light microscopy (LM). It was shown that PEO/PVA blend hydrogels did not induce any acute general toxic effects on mice and an allergic reaction for rabbit skin. The cell growth inhibition and morphology at the LM level were the very same as those of the control culture. To evaluate the healing effect of the blend hydrogels on burn, the hydrogels covered a wound formed on the back of rats. As compared with the corresponding gauze dressing group, the healing under the wet environment of the hydrogel dressing had some advantages: the wound healing is faster(p <0.01); it is easier to change the dressing without any damage to the regenerated surface and no dressing material remains on the wound. It is concluded that PEO/PVA blend hydrogel films may be considered as truly biocompatible, and the wet environment formed by the hydrogels is effective for fast healing of wound.  
        关键词：PEO/PVA hydrogel;Wound dressings;Toxicity;Healing effect   
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        发布时间：2023-04-07
      • JIA Shaojin,Zhang Zhicheng,XU Xianglin,GE Xuewu,YE Qiang,WANG Feng
        Vol. 18, Issue 2, Pages: 117-123(2000)
        摘要：Radiation-crosslinking improves a number of essential properties of polymers, so the high -temperature-resistance of polyethylene(PE) was increased by irradiation. The national products of High -density-polyethylene (HDPE), crosslinking promoters, flame-retardant, antioxidants, Si rubber, and Ethylene-propylene-monomer(EPDM) were used as cable insulation. After -irradiation, thermal-endurance can get above 135, and high flame retardacy was firmed, Oxygen index can get above 32 by crosslinking.  
        关键词：Flame retardant;Si Rubber;Polyethylene(PE);γ-ray irradiation;Crosslinking;Thermal stability   
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        发布时间：2023-04-07
      • LU Jun,ZHAI Maolin,YI Min,HA Hongfei
        Vol. 18, Issue 2, Pages: 124-128(2000)
        摘要：Graft polymerization of N-isopropylacrylamide (NIPAAm) onto cotton cellulose preirradiated in air by 60Co γ rays was accomplished in mixed solvents of water and alcohols or acetone. The graft yields changed with different ratios of water and organic solvents. In the system of solvents comprised of water and methanol, the Trommsdorff effect appeared in the curve of the graft yields vice verse the concentrations of the monomer. In the same system, the dependence of the graft yield of NIPAAm on the graft temperature was different from that of Acrylamide (AAm). The relation between the graft yields of the grafted products and their crystallinities measured by X Ray Diffraction could be regarded as a proof of the 'third phase' conception.  
        关键词：Cotton cellulose;N-isoprropylacrylamide;Preirradiation graft polymerization;Trommsdorff effect;'Third phases'   
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        发布时间：2023-04-07
      • ZHOU Jianhua,SU Liaoyuan,TONG Jian
        Vol. 18, Issue 2, Pages: 129-132(2000)
        摘要：The effects of γ-rays irradiation on CD3, CD4, CD8, B7.1 molecules expression of human lymphocytes which were irradiated with 0.5, 2, 4 and 8Gy γ-rays or with 0.5, 2, 4 and 8Gy (D2) γ-rays 4h after 10cGy irradiation (D1) were investigated in normal donor. 24h or 48h after final irradiation the changes of CD3+, CD4+, CD8+, B7.1+ molecules were measured by flow cytometry(FCM) with immunofluorescence technique. The results showed that the CD3+, CD4+, CD8+ molecules expression in the highly irradiated groups decreased with dose increase, while the CD3+, CD4+, CD8+ molecules privously exposed to 10cGy irradiation could reduce this influence, and the B7.1+ molecule expression in the high dose irradiation groups was enhanced with increasing dose.  
        关键词：γ-ray;CD3, CD4, CD8, B7.1 molecule expression   
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        发布时间：2023-04-07
      • ZHU Hanneng,MO Suzhen,XIONG Sidong,CHENG Wenyin
        Vol. 18, Issue 2, Pages: 133-137(2000)
        摘要：Telomerase has been detected in more than 85% of human tumor cell lines and tissure, but not exist in majority of nomal cell and tissure. PCR and TRAP were employed in this experiment to test the telomerase activity changing of tumor cell line Hela, A431 and KB after irradiated.The result indicated that:tumor telomerase activity and expression of hEST2 functional area fragment are dose-dependent decreased;the result of PCR test method are corrlated with that of TRAP, those data suggest that the supression effect of irradiation onto tumor telomerase activity is one of critical mechanism of radiotherapy.Determining the telomerase activity level perhapes is a new index to evaluate the killing effect of irradiation onto tumor.  
        关键词：Irradiation;Telomerase activity;PCR;TRAP   
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        发布时间：2023-04-07
      • LI Qiang,ZHOU Guangming,WANG Jufang,LI Wenjian,WEI Zengquan
        Vol. 18, Issue 2, Pages: 138-141(2000)
        摘要：Survival curves of Chinese hamster V79 cells exposed to accelerated carbon ions with linear energy transfers (LET) of 125.5, 200 and 700keV/µm were measured, respectively. Inactivation cross sections corresponding to the irradiation above were deduced from the survival curves of exposed V79 cells. They are 7.86±0.17, 10.44±1.11 and 32.32±3.58µm2, respectively in turn. With the survival response of V79 cells to 60Co γ rays as a reference value, relatively biological effectiveness (RBE) at 10%, 20%, 50% and 80% survival levels were given for the carbon ions. The results show that carbon ions with LET of 125.5keV/µm have higher values of RBE at any survival levels than others. It prompted that the maximum value of RBE for V79 cell surviving as the biological endpoint appears at the LET below 200 keV/µm for carbon ions.  
        关键词：Carbon ions;V79 cells;Inactivation cross section;Relatively biological effectiveness   
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        发布时间：2023-04-07
      • ZHANG Hao,MAO Bingzhi,LUO Qinglang,XIA Zhenbiao,DONG Bo,CONG Yuwen,GUO Dehuang
        Vol. 18, Issue 2, Pages: 142-147(2000)
        摘要：This article is to study the rehabilitate effects of G-CSF on hemotological function and its mechanism. The results indicate that G-CSF can not only accelerate the recovery of peripheral blood, promote the recovery of endogenous CFU-S, but also increase the number of bone marrow cell and stimulate its CFU-GM. It can raise the 30th days survival rate of irradiated mice. The mechanism is due to accelerative action on the recovery of hematological function in exposed mice. This procedure needs a long time. It is not due to the inhibitory upon programmed cell death of cells.  
        关键词：Cytokine;G-CSF;Irradiation   
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        发布时间：2023-04-07
      • DENG Pengyang,XIE Hongfeng,DENG Mingxiao,ZHONG Xiaoguang
        Vol. 18, Issue 2, Pages: 148-152(2000)
        摘要：By using DSC, the behavior of second melting and nonisothermal crystallization of pure gel pure sol and sol — gel blend of radiation crosslinking HDPE was studied. We found that, because of the existence of sol, there is notable difference between pure gel and pure sol or sol — gel blend. Under the same dose, the melting point and crystallization temperature of pure sol and sol — gel blend are higher than that of pure gel. At the same time, we also found that the Avrami exponent of original PE、pure sol and sol — gel blend is the similar to each other and different to that of pure gel, which means that the procedure of nucleation and growth of these samples is the same and also different to that of pure gel.  
        关键词：Radiation crosslinking;Polyethylene;Pure gel;Melting property;Nonisothermal crystallization kinetic   
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        发布时间：2023-04-07
      • ZENG Heping,DU Yunwu,JIANG Huaiying
        Vol. 18, Issue 2, Pages: 153-156(2000)
        摘要：This paper reports a successful application of the technology of the high-energy 8MeV electron irradiation in the manufacturing process for the High Power Fast Thyristor.  
        关键词：Electron irradiation;Fast thyristor;The lifetime of minority;Turn-off time;On-state peak voltage drop   
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        发布时间：2023-04-07
      • GUO Dehuang,DONG Bo,WEN Gengyun,LUO Qingliang,MAO Bingzhi
        Vol. 18, Issue 2, Pages: 157-160(2000)
        摘要：In order to explore the variety of cell proliferation and cell cycle after exposure to ionizing radiation, the responses of irradiated KG-1 cells of the human myeloid leukemia stimulated by GM-CSF, the most common used cytokine in clinic, were investigated. The results showed that GM-CSF enhance KG-1 cells prolifration, reduce G0/G1 block, increase S phase and G2/M phase. The stimulation effects of the GM-CSF is more effective in irradiated group than in control group.  
        关键词：Radiation;KG-1 cell;GM-CSF;Cell cycle   
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        发布时间：2023-04-07

        研究论文

      • ZHENG Xiulong,MENG Xiangshun,ZHAO Fang,LI Yu,GAO Jianguo
        Vol. 18, Issue 2, Pages: 91-95(2000)
        摘要：Abstract The aim of this study was to see whether Sodium Glycididazole （CMNa） could enhance the in situ tumor efficacy of radiation, using 4 different tumor models bearing mice. The results showed that in comparison with the irradiated group alone, CMNa could decrease the velocity of tumor growth and increase the days of Tumor Growth Delay（TGD） evidently in all four tumor types. CMNa showed radiosensitizing activity for all tumors with SERs greater than 1.3. It demonstrates that the novel radiosensitizer can potentiate the in situ anti-tumor efficacy of radiation and has different radiosensitization effect among the tested tumors. Whether the new radiosensitizer CMNa has any effects on normal tissue such as radiosensitive bone marrow was tested. Survival of bone marrow stem cells(CFU-GM） due to radiation alone or in combination with the drug was assessed. Nevertheless it did not enhance the radiosensitizing effect on normal bone marrow and SER=1. The Therapeutic Gain Factor（TGF） defined as SER of tumor divided by SER of normal tissue bone marrow is greater than 1.3. Maybe CMNa will be an effective new type radiosensitive drug for clinical cancer therapy.  
        关键词：KEYWORDS Novel hypoxic radiosensitizer;Sodium Glycididazole;Four tumor models;CFU-GM   
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        发布时间：2023-04-07
      • WANG Yuexing,ZHANG Hongyuan,WANG Lanjin,PAN Yan,FAN Zhengping
        Vol. 18, Issue 2, Pages: 96-103(2000)
        摘要：Abstract Combined effects on rabbits induced by γ-rays irradiation in combination with inhalation of benzene, toluene and carbon monoxide and the interactive characteristics （antagonistic, additive or synergistic） of these agents were studied. Ninety healthy male rabbits were equally divided into 9 groups, eight of which were test groups assigned according to four-factor- two-dose-level orthogonal layout [L8（27）] program and one was the control. The two dose-levels of the four factors were: for γ-rays irradiation, 0.0075Gy/d and 0.0375Gy/d, and for benzene, toluene and carbon monoxide, 40±15mg/m3 and 182±33mg/m3, 90±30mg/m3 and 407±68mg/m3, 93±4mg/m3 and 278±8mg/m3 respectively. The animals were exposed to γ-rays irradiation combined with benzene, toluene and CO vapor 2h a day and 5days a week for a period of 8 weeks. Experimental results were analysed with the method of variance analysis and the test groups were compared. The results showed that （1） the interactive factor, the ratio（ω） of the combined effects caused by the four agents to the sum of the four independent effects induced by those agents, was 2.16, 1.58, 2.07, 2.67, 1.31 and 1.18 for dicentric + ring, acentric, aberration cells, total aberration, micronuclei and SCEs/C in lymphocytes respectively, and the ratio for aberrant sperms was as high as 5.97. The ratios showed that interactions were mainly synergistic （ω>1）. However, for acentric interactions between γ-rays and benzene was antagonistic. （2） The four agents could all obviously cause decrease in testis weight index, and γ-rays, benzene and CO could markedly reduce the number of sperms and increase the ratio of aberrant sperms. The interactions between γ-rays and benzene for the decrease in testis weight index and for the elevation of the ratio of aberrant sperms were synergistic. （3） CO could cause decrease in total amount of leucocytes, thrombocytes and hemoglobin, and toluene cause decrease in lymphocytes. （4） Gamma rays irradiation combined with inhalation of benzene, toluene and CO could produce significant interactions which were mainly synergistic, and CO, when combined with γ-rays, benzene and toluene, could cause obvious effects on gland.  
        关键词：KEYWORDS γ-rays;Benzene;Toluene;CO;Combined effect;Interactive factor   
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        发布时间：2023-04-07
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