最新刊期
期 3 ， 2001
- 摘要：The UV/EB curing technology is developing fast due to the advantages of the physical properties of the products, technology and the benefit to environmental protection. In this article, the markets of different segments in the global main regions in recent years are summarized. The new applications of UV/EB technology developing rapidly are also described.关键词：Ultraviolet;Electron beam;Radiation curing;Market;Application
- 2
- |
- 0
- |
- 4
发布时间：2023-04-07 - 摘要：Changes of apoptosis and cell cycle progression of HeLaS3 cells following irradiation with different doses of X rays were observed. The percentages of apoptotic cells were examined by flow cytometry (FCM) after the cells had been stained with propidium iodine (PI) and Hoechst33342(HO),and the cell cycle was detected by FCM stained with only PI. It was found that the percentages of apoptotic cells and the cells in S phase and G2/M phase increased after irradiation with 0.5—4.0Gy X rays. So irradiation may induce apoptosis and cell cycle block for HelaS3 cell lines.关键词：Ionizing radiation;Apoptosis;Cell cycle;HelaS3
- 2
- |
- 0
- |
- 1
发布时间：2023-04-07 - 摘要：The changes in cyclin B1 and cdc2 transcription level of splenocytes in mice were observed after WBI with low and high doses of Xrays. The results showed that cyclin B1 mRNA expression slightly increased at 2h and 12—24h after 75mGy X rays, being 1.25, 1.27 and 1.22 times of sham—irradiated group, returning to sham—irradiated level at 48h. However, cyclin B1 mRNA began to decrease at 4h after irradiation with 2.0Gy X rays,reaching its lowest level at 12h, with a decrease of 39%, and returning to the level of sham—irradiated control. In addition, we observed the time course of cdc2 mRNA expression. The results showed that there were no changes in cdc2 mRNA expression at 2—48h after 75mGy WBI, but the time course of cdc2 mRNA expression with 2.0Gy WBI was the same as cyclin B1 after the same dose. The results indicated that low dose irradiation could induce an increase in cyclin B1 transcription level, accelerating the progression of cell cycle, but did not affect the expression of cdc2 gene. In contrast,high dose irradiation could induce the decrease of above two factors,with marked G2 arrest关键词：X rays;Splenocytes;Cyclin B1;cdc2 transcription level
- 2
- |
- 0
- |
- 0
发布时间：2023-04-07 - 摘要：Single cell microgel electrophoresis(SCGE) and MTT assay were used to determine the relationships between radiation—induced initial DNA double—strand breaks(DSBs), the ability of cells to repair DSBs and cellular radiosensitivity in both AT5BIVA and GM637 cell lines. The results demonstrated that AT5BIVA cell line was significantly more radiosensitive than GM637. The initial DSBs in both AT5BIVA and GM637 lines increased with the rise of irradiation dose,and showed significant dependence on dose. At a given dose of 20Gy, radiation—induced initial DSBs in AT5BIVA cell line was signficantly higher than those in GM637. The ability of AT5BIVA cell line to repair DSBs was more powerful than that of GM637. The results suggested that the radiation—induced initial DSBs in cells and the ability of cells to repair DSBs both correlated with cellular radiosensitivity to a certain extent, and could be used as potential predictors of intrinsic radiosensitivity of cells.关键词：Single cell microgel electrophoresis;DNA double—strand breaks;Repair;Radiosensitivity
- 4
- |
- 0
- |
- 0
发布时间：2023-04-07 - 摘要：To investigate the effect of ionizing radiation on the CDK4 expression in mouse bone marrow cells, Flow cytometry (FCM) and Northern blot were used to analyze the changes in the level of CDK4 mRNA and protein expression respectively after whole body irradiation(WBI) with X rays. The results indicated that both the levels of CDK4 mRNA and protein expression decreased significantly from 2h to 72h after 2Gy X rays. Meanwhile, we found a dose—dependent relationship for these two levels at 12h after irradiation with different doses (0.5,1, 2,4,6Gy). These results suggested that the effect of ionizing irradiation on CDK4 expression maybe have profound significance and desire further research.关键词：CDK4;Ionizing radiation;Bone marrow cells;G1 arrest
- 2
- |
- 0
- |
- 0
发布时间：2023-04-07 - 摘要：In order to estimate the influence of the un—uniform dose distribution on the clinical treatment result, the Influence of dose distribution homogeneity on the tumor control probability was investigated. Basing on the formula deduced previously for survival fraction of cells irradiated by the un—uniform heavy—ion irradiation field and the theory of tumor control probability, the tumor control probability was calculated for a tumor mode exposed to different dose distribution homogeneity. The results show that the tumor control probability responding to the same total dose will decrease if the dose distribution homogeneity gets worse. In clinical treatment, the dose distribution homogeneity should be better than 95%.关键词：Heavy—ion;Dose distribution homogeneity;Tumor control probability;Tolerance of normal tissues
- 2
- |
- 0
- |
- 1
发布时间：2023-04-07 - 摘要：To investigate the effect of bone morphogenetic proteins (BMPs) on the acute hematopoietic sickness induced in mice. BMP, rhBMP —2m and PBK/ hBMP —2 — NIH3T3 cells were prepared as previously discribed. In this study,the effect of BMPs on hematopoiesis included some hematological parameters. The survival ratio on the 30th d post irradiation and formation of bone marrow CFU — GM colomies were detected. The experiments indicate that pbBMP (purified bovine bone morphogenetic protein) could increase the formation of bone marrow CFU—GM colomies (p<0.05) at 10 th d after irradiation. rhBMP—2m increased the survival rate of mice irradiated by 7.5Gy. All mice in control group died in 30dayS, while 10%, 15%and 35% mice survived when they were injected ip with 0.5mg, 1.0mg and 2.0mg of rhBMP—2m respectively. All hematological parameters of treated mice were significantly higher than those of control group (p<0.01). PBK/ hBMP—2—NIH3T3 cells were established and transplanted into mice irradiated by 7.0Gy γ ray by i. p. The survival r atio of treated mice higher than that of negative control group (p<0.01), and all hematopoietic parameters were increased statistically significantly (p<0.01). These data supported our hypothesis: BMPs can be used for the tratement of the acute radiation sickness. The results indicated that in adult mice, BMPs could recover the hematopoietic injury from acute radiation sickness.关键词：Bbone morphogenetic proteins;Acute radiation sickness;Hematopoiesis
- 2
- |
- 0
- |
- 4
发布时间：2023-04-07 - 摘要：To investigate the effect of mouse marrow stromal cells on the growth of granulocyte—macrophage progenitor cells (CFU —GM) from mice with radiation injury. Semi—solid culture of CFU—GM on the confluent adherent stromal cell layers of marrow cells was adopted. The experimental data demonstrated that the numbers of nomal marrow CFU—MGM formed on stromal cell layers from mice with radiation injury were significantly reduced as compared with those from normal mice within 10d, especially at 3th and 5th day after injury. The supporting function of bone marrow stromal cells on the growth of CFU—GM was impaired under the irradiation conditions of which must not be neglected in clinical treatment of radiation—in¬ duced hematopoiesis dysfunction.关键词：Radiation injury;Bone marrow stromal cell;Granulocyte—macrophage progenitor cell
- 2
- |
- 0
- |
- 0
发布时间：2023-04-07 - 摘要：After irradiation with 0.5, 2,4 and 8Gy γ rays, CD28 and Fas (CD95)molecule expression in human T lymphocytes were measured by flow cytometry (FCM) with direct immunofluorescence technique. Before and after treatment of T cells with CD28McAb and IL—18, the T cells apoptosis was determined by JAM test and FCM technique. Results showed that with the increasing radiation dosage, the CD28 molecule expression decreased and the CD95 molecule expression increased in CD3+T cells, which could affect signal transduction and increase cell apoptosis. However,after using CD28 McAb and IL—18 to treat the cells irradiated, Fas expression and T cell apoptosis decreased.关键词：CD28;Fas;Ionizing radiation;Cell apoptosis
- 2
- |
- 0
- |
- 1
发布时间：2023-04-07 - 摘要：The aim of this study was to investigate the effect of radiation on the expression of interleukin — 6 in the bone marrow stromal cells (BMSCs) and to discuss the mechanisms by which the expression of interleukin — 6 increased. The BMSCs from normal Kungming mice were cultured in vitro with the method of Dexter. The expression of interleukin — 6 on the levels of protein and mRNA were detected by the uses of radio—immunity assay(RIA) and the reverse transcription polymerase chain reaction(RT—PCR) respectively. PDTC and dexamethasone, two different inhibitors of nuclear factor—kappa B, were used as controls. The result of RIA showed that the expression of interleukin—6 were distinctly increased at 1 hour, with its peak at 4 hours post irradiation in the supernatant of culture of murine BMSCs when the BMSCs were irradiated by 8.0 Gy 60Co γ rays. The results of RT—PCR showed that the expression of IL—6 mRNA reached its peak at 8 hours. After adding NF—kappa B inhibitor in the supernatant before radiation, there was no difference between control group and radiation group in detecting the mRNA of IL — 6 using RT—PCR. This study suggested that the transcription and expression of IL—6 were activated by ionizing radiation, which might be due to the reason that ionizing radiation could activate NF—kappa B.关键词：Ionizing radiation;Bone marrow stromal cells;Interleukin—6;NF—kappa B
- 2
- |
- 0
- |
- 0
发布时间：2023-04-07 - 摘要：To study the role of
- 2
- |
- 0
- |
- 2
发布时间：2023-04-07 - 摘要：In order to improve the treatment of functional disorders with result from the clinical radiotherapy of encephalic diseases with rotate head γ knife,the pathological changes and expression of TNF—α and IL—1β mRNA in the right caudate nucleus of the rat brain irradiated locally with a single dose of 200Gy γ rays were studied by general and electron microscopical observation and in situ hybridization respectively. The results showed that the pathological changes develop through 4 stages: (1) cerebral edema (0—14 days after irradiation) ; (2) necrosis of the injured tissue (21d — 2 months); (3) scavenging of the necrotic tissue (3 — 6 months) and (4)hyperplasia stage (9 —12 months). It was also found that the permeability of the blood —brain barrier began to increase 2 hours after irradiation,and the degree of increase showed a positive correlation with the survival period of the animals. The ultrastructural changes in endotheliocytes and the gliocytes occurred prior to that of neurons. In this study,the expression of TNF—α and IL —1β mRNA was found increased in irradiated brain tissue, and treatment of rats with dexamethasone before irradiation could inhibit this expression.关键词：Gamma knife;Radiation;Rat;Brain necrosis
- 2
- |
- 0
- |
- 1
发布时间：2023-04-07 - 摘要：Low energy N+ ions were produced by gas arc discharge and the ions were accelerated into cytosine (C) aqueous solution. Changes in pH, UV, FT—IR and 1H—NMR spectra of C solution after this kind of ions'action were investigated. It was showed that pH of the solution decreased with N+ implantation,N+ caused various damages on C molecules and many new substances were founded. These new substances were synthesized not only by combination of the damage fragments in the solution, but also by "deposition" of N+ from outside.关键词：Arc discharge;Low energy ions;Cytosine aqueous solution;Radiation damage
- 2
- |
- 0
- |
- 1
发布时间：2023-04-07 - 摘要：Male Wistar rats were exposed to 0, 10, 20, and 30 working level months(WLM) of radon and its progeny by inhalation. DNA damage of rat peripheral lymphocytes induced by radon and progeny in vivo was investigated by using 3H—TdrR incorporation assay and alkaline single cell gel electrophoretic technique. The CPM value of rat peripheral lymphocytes from radon exposed groups was decreased significantly compared with the control,while the tail movement of lymphocytes gel electrophoresis was increased significantly, with a dose—effect relationship. The results suggest that radon and its progeny can suppress DNA synthesis of lymphocyte, reduce transformation ability of lymphocyte and damage immune function,which are useful implications in radiation protection against radon toxicity.关键词：Radon and its progeny;Lymphocytes;DNA damage
- 2
- |
- 0
- |
- 1
发布时间：2023-04-07
- 摘要：LDR could stimulate lymphocyte transformation for adults, children and infants. The effeet of LDR on lymphocytes in malnourished children was lower,but higher on lymphocytes in cord blood. The effect of LDR on
- 2
- |
- 0
- |
- 2
发布时间：2023-04-07
研究论文
- Technical support is provided by Beijing Founder electronics co., LTD 沪ICP备05005479号-1
京公网安备11010802024621 - It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
- Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
0