最新刊期
      期 4 ， 1989
      • Huang Guanglin,Chen Zefang,Li Yunhui,Zhong Yaoqiong,Xiong Wenshu,Zhou Wei
        Vol. 7, Issue 4, Pages: 1-7(1989)
        摘要：The influence of unsaturation of various polyethylene on the radiation cross-linking, with and without polyfunctional monomers, was investigated. The results showed, that the suspended double-bonds of vinylidene has higher contribution to the radiation cross-linking. The polyethylene contained a bit more double-bonds possessed auto-sensibilized cross-linking action. The sensibilizing agent added outside posses has very small influence on the acceleration of radiation cross-linking.The effects of three autioxidants (1010, 3114 and PG) on the radiation cross-linking efficiency of LDPE were studied. Experimental results showed, both 1010 and 3114 decreased the amount of crosslinking at a given dose, contrarily, the PG increased it. PG decomposed by radiation and produced OH radical which could accelerate the radiation cross-linking.When the polyfunctional monomer was added to the system, the radiation cross-linking of LDPE containing 1010 was accelerated. However, for sample cotaining PG the sensibilizer exhibited very small influence on the radiation cross-linking.  
        关键词：Polythylene;Radiation cross-linking;Sensibilizer;Unsaturation;Antioxidant   
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        发布时间：2023-04-23
      • Wu Zhili,Chen Xinwei
        Vol. 7, Issue 4, Pages: 8-13(1989)
        摘要：Two kinds of the dose labels, SN1 and SN2, developed by our laboratory are the yellow plastic. During irradiation to different dose using cobalt-60 gamma-ray, the color of labels turns from yellow to orange or red. Initial dose to change in to red due to irradiation are<1kGy for SN1-1 and SN2-1, 1-10kGy for SN1-2 (or3) and SN2-2(or 3), >10kGy for SN1-4 and SN2-4. In this paper using visual colorimetry, color reflection densitometry and test of the color difference the characteristics of the labels have been investigated. Color development due to irradiation is not affected by dose rates varying between 3.6 and 100Gy/min. No effect on color development is oberved with humidy between 12 and 97%, with temperature between 5 and 55℃. The labels is not sensitive to ambient light, and shelf life is over 3 years under conditions of laboratory storage. The color of labels after irradiation can satisfy archival requirements in "go-no go" . The labels of SN type can provide a visual means of determing whether a plant package was irradiated in proces, and can identify whether inventory product were processed, but theirs can give a visual indication of the dose regians of recive radiation in a plant package. These labels may cover a range from 0.1 to 100 kGy.  
        关键词：Radiation processing;Dosimetry   
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        发布时间：2023-04-23
      • Xu Guanfan,Luo Yunxia
        Vol. 7, Issue 4, Pages: 14-18(1989)
        摘要：Our XPS studies of some fluoropolymers have shown that the cleanliness of specimen surfaces is very important for obtaining good results. Data will not be reliable if the specimen surfaces to be studied are contaminated. It is found that the contaminants can basically be removed with some organic solvents, in which tetrahydrofuran is most efficient.  
        关键词：Fluoropolymer;Surface cleanliness;XPS   
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        发布时间：2023-04-23
      • Zhu Shoupeng,Zheng Siying,Wang Liuyi,Yang Shujin
        Vol. 7, Issue 4, Pages: 19-24(1989)
        摘要：The purpose of the present study is to ascertain comparative retention of fission fragment 147Pm in regenerated and fetal liver on induction, of chromosome aberrations in these cells. The results indicated that retention of 147Pm in regenerated liver was about 700 times than in fetal liver. The cumulative absorption dose in regenerated liver was about 2.87Gy, while in fetal liver — only 0.004 Gy. Under the same conditions, the incidence rate of chromosome aberrations in regenerated liver cells induced by 147Pm was 50.2%, and in fetal liver cells — about 28.3%. It should be concluded that the radiosensitivity to 147Pm was not uniform among the regenerated and fetal liver cells. Our study suggested that fetal liver cells show to be more radiosensitive to 147Pm than regenerated liver cells. Among the type of aberrations in both cells induced by 147Pm, chromatid breakages were predominant, accompanied with a few chromosome breakages.  
        关键词：147Pm;Retention;Regenerated liver cells;Fetal liver cells;Mutagenic effect   
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        发布时间：2023-04-23
      • Zhang Yangpei,Bai Xiaobin,Chen Yueneng,Xu Huiying,Fan Guocai,Xia Shouxuan
        Vol. 7, Issue 4, Pages: 25-29(1989)
        摘要：The DNA-mediated gene transfer had been carried out by both Calcium phosphate coprecipitation and electroporation method. The cellular DNA and DNA fragments from human cervical carcinoma HeLaS3 cells were introduced with PSV2Neo DNA into XP20S (SV40) cells. The transfectants were picked up after twice selections by G418 and 3J/m2 UV-irradiation. The results showed that cellular DNA and Bgl Ⅰ, Xho Ⅰ digested DNA fragments from HeLaS 3 could correct the deficiency of excision repair gene in XP cells, and cause the recipient cells resistant to UV irradiation. The second transfection experiment confirmed that HeLaS3 DNA were really integrated into XP cell chromosome and stably expressed within the cell genome.  
        关键词：UV-irradiation;Excision repair;Gene transfer;XP cell;HeLaS3 DNA   
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        发布时间：2023-04-23
      • Geng Yongzhi,Su Liaoyuan,Liu Keliang
        Vol. 7, Issue 4, Pages: 30-35(1989)
        摘要：The transformation, DNA single strand breaks and its repair in human peripheral blood lymphocytes stimulated with PHA, ConA and PWM respectively following exposure to 60Co γ-rays were analyzed by 3H-thymidine incorporation and hydroxylapatite chromatography. The transformation of lymphocytes stimulated with PHA, ConA and PWM were suppressed by γ-rays and the dose-effect curves were biphasic within the range of 0-8 Gy. The lymphocytes stimulated with PWM were the most resistant to γ-rays. The extent of DNA single strand breaks in lymphocytes induced by γ-rays was linearly related to the dose within the range of 0-30 Gy and there was no marked difference in the three kinds of lymphocytes. After a period of post-irradiation (15 Gy) incubation at 37℃ the DNA single strand breaks could be rejoined but incompletely. The rejoined strands broke again if the cells were incubated longer. The repair ratio of single strand breaks in the lymphocytes stimulated with PWM was the highest among the cells with three mitogens. The results suggest that the difference of the radiation effect on transformation in different lytphocytes is probably related to the repair ability of DNA single strand breaks.  
        关键词：Lymphocyte;DNA single strand break;DNA repair;Radiosensitivity;PHA;ConA;PWM   
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        发布时间：2023-04-23
      • Zhang Zhonglun,Zheng Yanzhen
        Vol. 7, Issue 4, Pages: 36-41(1989)
        摘要：Low limits of measuring dose are limited by self-glow of luminol solution and luminescence of unradiated samples of saccharides. The suitable concentration of luminol soolution should be 7×10-8 mol/ml and a high ratio of signal to noise can be obtained when solution temperature is in the range of 15-25℃ The ranges suitable for dose measurement have been evaluated based on dose responce characteristic of these saccharides. Sensitivities are in this order of priorities: Trehalose, sucrose, galactose, glucose, levulose, mannose, and dose measurement dispersion change to poor according to this priorities also. Stability of lyoluminescence of the saccharides is not depended on heat treatmant of the samples.  
        关键词：Lyoluminescence dosimetry;Saccharide;γ-ray dosimetry   
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        发布时间：2023-04-23
      • He Weishun,Liu Aihua,Lin Shiying,Xiong Xikun
        Vol. 7, Issue 4, Pages: 42-46(1989)
        摘要：The rape pollen contains many biologically active and nutritive substances that are easily spoilt by bacteria because there are abundant nutritive substances. In the present study on disinfection, the rape pollen was irradiated with 2.5 kGy 60Coγ-ray. We used micronuciei, sister chromatid exchanges (SCE) of bone marrow cells and chromoscmal aterrations of meiotic cells in mice as an indicater of chromosomal damage to study the mutagenicity of irradiated rape pollen. The results are as follows:(1). Micronuclei: The frequency of micronuclei in polychromatic erythrocytes is 2.00‰; nucleated cells is 0.85‰ in control group. In the numbers of polychromatic erythrocytes and nucleated cells with micronuclei, there is no obviously difference in irradiated and unirradiated groups.(2) SCE: SCE incidence of control group is 2.01±0.12 /cell. No significant difference in the frequency of SCE exists between non irradited rape pollen and the control groups. But the frequency of SCE in irradiated rape pollen group (3000 mg/kg/day×7) is 2.36±0.12/cell; high dose group (6000 mg/kg/day×7) is 2.96± 0.14/cell. In comparison with control group, there is significant difference.(3). The meiotic chromosomal aberration: The chromatid breaks, fragments, and univalents in primary spermatocytes have been obseved. The frequencies of chromosomal aberration showed no obviously difference among irradiated and non irradiated rape pollen groups. The univalent frequencies of X-Y chromosomes in the experimental groups didn't found the difference with the control group which is 9.2% and ovre similar to that of the reference.  
        关键词：Rape pollen;Radiation;Mouse;Chromosomal damage   
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        发布时间：2023-04-23
      • Wang Chongdao,Lin Xingcheng,Zhu Yuyu,Xu Changshao,Yao Daoyuah,Gao Yaoming,Yu Zhiying
        Vol. 7, Issue 4, Pages: 47-49(1989)
        摘要：This paper describes the changes of LPO contents of some tissues in rats before and 72 hr after whole-body radiation with single 10 Gy dose of γ-irradiation and of serum LPO levels in 22 patients with malignant tumours before and after radiotherapy with 64-150 Gy total dose of fractional local γ-irradiation. LPO in the animal tissues and the human sera were determined by the modified spectrophotometry of TBA.The results showed that LPO contents of liver, spleen, brain and kidney in rats dramatically increased after radiation, and among them the change of LPO of spleen was the most remarkable. There was a marked difference between LPO levels in patients with malignant tumours before and after radiation exposure.  
        关键词：LPO;Rats;Patients with malignant tumours;Modified spectrophotometry of TBA   
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        发布时间：2023-04-23
      • Wang Chuanshan,Xu Mengjie,Li Zenlian,Feng Yongxiang,Li Panlin
        Vol. 7, Issue 4, Pages: 50-52,41(1989)
        摘要：Energy deposition in multilayer medium and the depth dose distribution in the layers are studied. Based on semi-empirical calculation of electron energy absorption in matter with EDMULT program of Tabata and Ito, further work has been carried out to extend the computation to multilayer composite material. New program developed in this paper makes IBM-PC compatible with complicated electron dose calculations.  
        关键词：Electron;Multilayer;Depth-dose distribution   
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        发布时间：2023-04-23
      • Tian Chenghui,Huang Jinzhong,Cheng Guodong,Zhang Rulin,Liu Jinwen
        Vol. 7, Issue 4, Pages: 53-56(1989)
        摘要：This paper reports the parallel observations of chromosomal aberrations frequencies and micronuclei frequencies in peripheral blood lymphocytes from 893 occupational exposure workers. The results show that the correspond with chromosomal aberrations frequencies and micronuclei frequencies are 7.84%. In view of theortical analysis, the correlative test is no a close relationship possibility between chromosomal aberrations and micronuclei frequencise. The distribution study of micronuclei in T. Blymphocytes for analysing relationship between the chromosomal aberrations frequencies and micronuclei frequencies has important theoretical significance and practical value.  
        关键词：Occupational exposure;Chromosomal aberrations frequencies;Lymphocytes micronuclei frequencies   
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        发布时间：2023-04-23
      • Zhu Bingchai,Lü Jiaben,Wang Congwu,Chen Tiehe
        Vol. 7, Issue 4, Pages: 57-59(1989)
        摘要：In this paper, combined effects of γ-ray radiation and high atmospheric pressure on chromosome aberrations, micronucleus and transformation frequency in peripheral blood lymphocytes have been studied. The results indicated that there were no significant influence for effects of high atmospheric pressure on chromosome aberrations, transformation frequency in peripheral blood lymphocytes induced γ-ray radiation, and that high atmospheric pressure increased effect of micronucleus in human peripheral blood lymphocytes in vitro induced γ-ray radiation.  
        关键词：High atmospheric pressure;Lymphocyte;combined effect   
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        发布时间：2023-04-23
      • Xu Yiming,Xu Guori,Yang Xianchun,Zhang Zhiyi
        Vol. 7, Issue 4, Pages: 60-62(1989)
        摘要：In this work, the photosensitive damages of three drugs of Porphyrins (YHPD, BHPD and Photofrin Ⅱ) on Calf Thymus DNA were compared by Laser Raman spectroscopy at molecular level. It is indicated that the damage of YHPD on Calf Thymus DNA is the strongest, and then the Photofrin Ⅱ, and the third BHPD.  
        关键词：Photosensitive drugs of Porphyrins;DNA;Photosensitive damages;Raman spectroscopy;Laser   
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        发布时间：2023-04-23
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