最新刊期
      期 6 ， 2006
      • WANG Hengdong,SUN Xieqing,YE Yin,QIU Shilong
        Vol. 24, Issue 6, Pages: 321-326(2006)
        摘要：Fluoride sulfonic proton exchange membrane has a much bright future for its high thermal stability, high chemical stability and good mechanical strength. In this paper, we prepared a fluoride sulfonic proton exchange membrane by irradiation induced grafting, and tested the membrane for its physical and chemical property such as tensile strength, area resistance and degree of base absorption, etc. The membrane was observed by SEM and tested in a proton exchange membrane fuel cell for further research.  
        关键词：Irradiation induced grafting;Proton exchange membrane   
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        发布时间：2023-03-30
      • CHEN Honghong,JIANG Xun,YU Lei,TAN Zhiqing,HU Yuxing,WANG Wenzhu,CHENG Wenying,SHAO Chunlin
        Vol. 24, Issue 6, Pages: 327-332(2006)
        摘要：Biodegradation properties of chitosan-coated PGLA (poly (lactic-glycolic acid)) brachytherapy seeding carrier was investigated by continuous low-dose-rate irradiation of 125I seeds in vitro. PBS (phosphate-buffered solution) was used to simulate internal environment in vitro. Five 125I seeds with an apparent activity of 29.6MBq each were arranged in coated PGLA carrier. Weight loss, elasticity recovery rate and SEM (Scanning electron microscope) observation were used to evaluate its biodegradation properties. The coated PGLA carrier was slightly degraded by losing the weight of 10% and elasticity retention rate still kept at above 60% after two week’s exposure. The PGLA filament structure was well maintained and only a few coating layers were detached. With a maximum dose of 2.2kGy in the coated PGLA carrier after four weeks’ exposure to the 125I seeds, the weight loss rate was less than 50% and elasticity retention rate was 36%. SEM observation indicated further detachment of coating layers and fissure and slit of a few filaments. The results showed that continuous low-dose-rate irradiation delivered by the 125I seeds caused to some extent accelerated biodegradation of chitosan-coated PGLA after four weeks’ exposure in vitro, but majority of mass and certain elasticity were retained and the morphology structure was well maintained. The chitosan-coated PGLA carrier could maintain the space distribution of 125I seeds.  
        关键词：125I seed carrier sheet;PGLA(Poly (lactic-glycolic acid));Chitosan;Biodegradation;in vitro   
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        发布时间：2023-03-30
      • FANG Chuanji,LU Zhaoxin,SUN Lijun,LV Fengxia,BIE Xiaomei
        Vol. 24, Issue 6, Pages: 333-336(2006)
        摘要：Bacillus amyloliquefaciens ES-2 was implanted with 20keV N+ ion beam to breed mutants of high yield of antimicrobial lipopeptides. The dose effects of the nitrogen ion implantation on the survival and positive mutations rate was studied. The mutant strain designated as B. amyloliquefaciens ES-2-4 showing higher yield of antimicrobial lipopeptides was obtained. The concentration of the lipopeptides in fermentation liquid increased by 15.2% compared with the original strain. We also studied the fermentation process between the mutant and the original strain. It indicated that the mutant with shorter lag phase, longer stable phase and higher yield of the lipopeptides, which was suitable for large-scale production.  
        关键词：Ion implantation;Antimicrobial lipopeptides;Mutation breeding;Fermentation   
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        发布时间：2023-03-30
      • LIU Meihua,DENG Pengyang,SUN Guoen,ZHANG Wanxi,SUN Jiazhen,DONG Lisong
        Vol. 24, Issue 6, Pages: 337-340(2006)
        摘要：The radiation effect of ethylene-vinyl alcohol copolymer (EVOH), EVOH/glycerin blend was studied by solvent extraction, gel permeation chromatography (GPC) and Fourier transform infrared spectrum (FTIR) methods. Samples were irradiated up to 1800kGy at room temperature under N2. The results show that degradation is the main reaction in pure EVOH. Trace gel content could be found in E151 irradiated to at least 800kGy, and only 5.9% gel content was found in the sample irradiated to 1200kGy.While trace gel content could be found in F101 irradiated to at least 1800kGy. The different gelation doses of E151 and F101 are due to different contents of vinyl alcohol units. Unsaturation structure can be found in the irradiated EVOH. The content increased at first, and then decreased, with the dose. The existence of double bond enhances the radiation efficiency of EVOH. For EVOH/glycerin blend, the gel content was than that of pure EVOH when the absorbed dose exceeds 800kGy, and the gel content increased with the absorbed dose. But it cannot enhance radiation efficiency of EVOH as water.  
        关键词：Ethylene-vinyl alcohol copolymer;Radiation effect;Gel permeation chromatography (GPC);Fourier transform infrared spectrum (FTIR)   
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        发布时间：2023-03-30
      • LI Zhenzhong,HE Wei,MA Yalin,ZHANG Wenxiong
        Vol. 24, Issue 6, Pages: 341-344(2006)
        摘要：Maleic anhydride grafted high density polyethylene (HDPE-g-MAH) were prepared by UV-irradiation. Factors of the irradiation time, monomer content, initiator content, and styrene as a co-monomer on the grafting rate and gel content of HDPE-g-MAH were studied. The results show that the optimal duration of UV-irradiation is 30s. The grafting rate increases with MAH content, while the grafting efficiency increases up to 5.2% MAH, where it drops. The content of HYPERLINK "http://www.htbc.cn/t_product_c_3.htm" benzophenone (BP) affects greatly the grafting rate and gel content, and the optimal BP content is 0.5%. Adding styrene into the graft system reduces apparently the gel content of HDPE-g-MAH.  
        关键词：Maleic anhydride;UV irradiation;Grafting;High density polyethylene   
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        发布时间：2023-03-30
      • GONG Peijun,HONG Jun,LIU Xingfen,YU Jiahui,FAN Chunhai,DONG Li,YAO Side
        Vol. 24, Issue 6, Pages: 345-350(2006)
        摘要：An improved approach for preparing amino-modified magnetic nanogels has been developed in this paper. Polyallylamine magnetic nanogels (PAA-MNGs) were synthesized in one step by photoinitiation and in situ photopolymerization of allylamine in Fe3O4 aqueous suspension under UV irradiation. Morphology, mean diameter and diameter distribution of the PAA-MNGs were measured by TEM and PCS respectively, while the magnetite content was confirmed by TG and the superparamagnetism identified by VSM. Biomolecules, such as proteins, can be coupled to the surface of PAA-MNGs in virtue of amino groups. Streptavidin-coated magnetic nanoparticles (SA-MNPs) were prepared by covalent immobilization of streptavidin (SA) activated by 1-Ethyl-3- (3-dimethyllaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysuccinimid (NHS) on PAA-MNGs. The biotin binding capacity of SA-MNPs was proved by colorimetric measurements using the probe biotin p-nitrophenyl ester (BNPE). Therefore, PAA-MNGs may be a suitable vehicle to carry biomolecules for applications in many areas of biotechnology.  
        关键词：Polyallylamine;Magnetic nanogels;UV irradiation;Streptavidin   
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        发布时间：2023-03-30
      • LIU Zhaomin,CHANG Liang,QIN Li
        Vol. 24, Issue 6, Pages: 351-355(2006)
        摘要：PLGA-PEG-PLGA triblock copolymer were synthesised with different compositions. Structure and molecular weight of the copolymer were characterized by FTIR, 1H-NMR and GPC. Phase diagram of the copolymer and their molecular weight variation under E-beam irradiation were studied. The results showed gelation of the copolymer synthesized in 8h at 150℃ with 0.04g catalyst, 5.08g glycolide, and 12.61g DL-lactide (molar ratio 1:2) had sharp sol-gel phase transition. In an aqueous solution of defined concentration of the copolymer with PEG of fixed molecular weight, by increaseing the DLLA/LA ratio and molecular weight of the copolymer, the temperature of sol-gel phase transition decreased, while the temperature of gel-sol phase transition increased. Maximum polydispersity could be achieved by irradiating the copolymer to 20kGy.  
        关键词：Block copolymer;Aqueous gela;Biodegradable;Thermoreversible;Phase transition;Irradiation   
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        发布时间：2023-03-30
      • SU Xiaoming,ZENG Guiying,REN Dongqing,TIAN Ruifeng,ZHOU Xiaoguang,ZHAO Tao,WANG Shuang,YANG Jiaji,XU Xiuli
        Vol. 24, Issue 6, Pages: 356-361(2006)
        摘要：The work was aimed at exploring protective effects of intestinal RNA on intestinal mucosal barrier in mice after abdominal γ-irradiation . The BALB/c male mice were abdominally irradiated with 11.50Gy 60Co γ-ray in 1—3h, and then they were injected intestinal RNA from normal on jejunum. On 1, 3 and 5d after irradiation, the mice were sacrified after anesthesia for determining sIgA in small intestinal mucilage, endotoxin in blood and bacterial metathetic rate, and observing morphological changes of jejunal villus. The result showed that intestinal RNA can decrease MLN bacterial metathetic rate and the level of endotoxin in blood, and increase sIgA in small intestinal mucilage (p<0.01). In addition, it can improve intestinal mucosal morphology and reduce atrophy and collapse of jejunal villus. In conclusion, Intestinal RNA can improve intestinal mucosal barrier and inhibit intestinal bacterial translocation and absorption of intestinal endotoxin in irradiated mice.  
        关键词：Intestinal RNA;γ-ray;sIgA;Endotoxin;Bacterial translocation   
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        发布时间：2023-03-30
      • JI Xinxin,HOU Wugang,ZHAO Jie,ZHAO Yong,LI Wei,ZHANG Yuanqiang
        Vol. 24, Issue 6, Pages: 362-366(2006)
        摘要：Changes in expressions of smad4, smad1 and smad5 in the spermatogenic cells of mice after whole-body exposure to electromagnetic pulse (EMP) were studied to elucidate possible mechanisms of the EMP-induced injuries. Twenty adult Balb/c mice were irradiated with 400kV/m EMP to the whole-body. Specimens were harvested on 1, 7, 14, 21and 28 days after the irradiation. Changes in cellular localizations and expressions of Smad4, Smad1 and Smad5 were examined by immunohistochemical ABC method. Quantitative analysis of the immunostaining was made by an image analysis system. In the seminiferous tubules, the localization of Smad4, Smad1 and Smad5 in the EMP group were significantly different from the control group. The expression densities of smad4, smad1 and smad5 changed significantly on 1, 7, 14, 21and 28 days after irradiation. The study indicates that Smad4, Smad1, Smad5 and BMPs/Smad signal pathway may play an important role in the process of impairment and repair of mouse testis irradiated by EMP.  
        关键词：Smad;BMPs;Electromagnetic pulse (EMP);Mouse;Testis   
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        发布时间：2023-03-30
      • ZHANG Zengli
        Vol. 24, Issue 6, Pages: 367-369(2006)
        摘要：Radioprotective effect of vitamin D on bone marrow stroma1 cells (BMSCs) was studied. Mice were administrated subcutaneously with a daily dosage of 1,25 dihydrxyvitamin D for three days from 48h prior to 6.0Gy gamma-irradiation until they were sacrificed. The number of CFU-F, the apoptosis rate of bone marrow stromal cell, and the expressions of caspase-3 in BMSC were detected. The number of CFU-F significantly decreased after irradiation and vitamin D administration preserved the number of CFU-F in irradiated mice at same level as control. The apoptosis rate and relative level of caspase-3 in BMSC were significantly increased after irradiation and vitamin D administration ameliorated this effect. These data suggest that vitamin D can protect BMSC from apoptosis induced by irradiation.  
        关键词：γ-irradiation;Bone marrow stroma1 cell;Vitamin D;Apoptosis   
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        发布时间：2023-03-30
      • SONG Weiqiang,SONG Qinghuan,TI Yongzhou,LV Xiaohua,WU Chengxun
        Vol. 24, Issue 6, Pages: 370-372(2006)
        摘要：Hyperbranched unsaturated polyesteramide (HUPEA) irradiated by 60Co γ-rays was blended into polycarbonate (PC) and mechanical properties of the blends were compared of the controls, i.e. pristine PC and PC with non-irradiated HUPEA. The results showed that blending PC with non-irradiated HUPEA or HUPEA irradiated to 50kGy had decreased tensile and flexural modulus, while PC blended with HUPEA irradiated to 100kGy increased the tensile and flexural modulus by 31.7% and 7%, respectively. The mechanisms of the mechanical properties change were discussed.  
        关键词：Hyperbranched unsaturated polyesteramide;Polycarbonate;Mechanical property;Irradiation   
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        发布时间：2023-03-30
      • XUE Jing,LIU Fenju,YU Jiahua,LI Bingyan,YANG Xueqin,SUN Zhiqiang
        Vol. 24, Issue 6, Pages: 373-377(2006)
        摘要：To study radiosensitizing effect and apoptosis induction of wortmannin on human glioma cells, colony formation assay, flow cytometry, laser confocal microscope were used to investigate the inhibition effect on colony formation, apoptosis and cell cycle procession. Experiment results suggested that wortmannin could inhibit colony formation in SHG-44 cells, and the combination of wortmannin with 60Co γ-irradiation showed a synergistic action. Wortmannin could also induce G1 arrest, apoptosis, and morphologyical changes such as karyopyknosis, nuclear-fragmentation and apoptotic body were observed. The above results revealed that wortmannin can sensitize SHG-44 cells to gama radiation, which might be related with G1 arrest and apoptosis pathway.  
        关键词：Wortmannin;Glioma;Radiosensitivity;Apoptosis   
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        发布时间：2023-03-30
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