最新刊期
      期 2 ， 2003
      • QU Bo,WU Jilan,WANG Wenqing
        Vol. 21, Issue 2, Pages: 81-82(2003)
        摘要：The research of radiolysis of dilute solutions of cyclohexane-phosphoryl compounds is an important subject, but the mechanism and rate of energy transfer are disputable. This paper focuses on whether the energy transition process is diffusion-controlled and excited cyclohexane has abnormal reaction-radium or not, etc.  
        关键词：Cyclohexane;Radiolysis;Energy transfer;Diffusion-controlled   
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        发布时间：2023-03-30
      • CHEN Xiaojing,JIN Yizun
        Vol. 21, Issue 2, Pages: 83-87(2003)
        摘要：Hypoxia is a feature that exists in most solid tumors. Bio-reductive drugs are pro-drugs that can selectively target the hypoxia cells in tumors. In reductive environment, they are reductively metabolized to generate toxic species. There are 3 main classes of bio-reductive drugs: the nitro-aromatics, N-oxides and quinones. Recently, bio-reductive drugs were combined with GDEPT for the treatment of cancer, in addition to radiation and the chemotherapeutic agents. bio-reductive drugs will play a significant role in future cancer therapy.  
        关键词：Bio-reductive drugs;Hypoxia;Reductive enzymes;Gene-directed enzyme pro-drug therapy (GDEPT);Nitro-aromatics;N-oxides;Quinones   
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        发布时间：2023-03-30
      • SU Liaoyuan
        Vol. 21, Issue 2, Pages: 88-90(2003)
        摘要：There have been growing interests in recent years over the effects of low doses of ionizing radiation on human. The paper gives a brief review on the LDR studies, which include LDR-induced induced hormesis and adaptive response, LDR experiments in vivo or in vitro and epidemiologic investigation, and clinical applications of LDR as well.  
        关键词：Low dose radiation;Hormesis;Adaptive response;Clinical application   
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        发布时间：2023-03-30
      • XIANG Qun,BAO Borong,CAO Weiguo,YANG Xingcun
        Vol. 21, Issue 2, Pages: 91-93(2003)
        摘要：N-octanoyl—2-methylpiperidine (OMPPD) a novel amide type extractant for the extraction of U (VI) from aqueous nitric acid medium, was studied for γ-ray irradiation effect. The effects of radiation dose, concentration of nitric acid and diluent on the distribution ratio for the extraction of U (VI) were studied. It is found that OMPPD has better radiation stability compared with Tributyl phosphate (TBP).  
        关键词：Radiation;N-octanoyl-piperidine;OMPPD;U (VI) extraction   
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        发布时间：2023-03-30
      • JIANG Bo,YANG Zheng,TANG Fangyuan,ZHOU Yong,HUANG Guanglin,LIN Libin
        Vol. 21, Issue 2, Pages: 94-98(2003)
        摘要：A study was carried out on radiation-induced cationic polymerization enhanced by the N-Alkoxypyridinium salt having relatively stable nonnucleophilic anion (PF6). After investigations on the cationic inhibitors, different atmosphere conditions and the molecular weight and its distribution, it was proved that the cationic polymerization to take place in CHO/ CH2Ｃl2 or C6H6/ EMP+PF6 system on irradiation with theγ-rays. The onium not only oxidated α-alkoxyalkyl radicals, produced from CHO in dichloromethane, to the correspondings cations, but also gave nonnucleophilic anions PF6 for the polymerization system. A preliminary discussion is made on the effect of different polar solvents to reactive centers.  
        关键词：Cyclohexene oxide;Pyridinium salts;γ-ray;Oxidation of radicals;Cationic polymerization;Radiation curing   
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        发布时间：2023-03-30
      • PAN Jingxi,LIN Weizhen,HAN Zhenhui,MIAO Jinling,WANG Wenfeng,YAO Side,LIN Nianyun,ZHU Dayuan
        Vol. 21, Issue 2, Pages: 99-101(2003)
        摘要：By use of laser flash photolysis and steady state absorption techniques, a systematic study was carried out on the interaction of ADE, which is a kind of model compound of actinomycin D, with DNA and BSA. Non-covalent binding was found between ADE and ssDNA、dsDNA and BSA, and photoinduced electron transfer reaction was observed. These results indicate that actinomycines might be used as type I photodrugs in the future and will be helpful for structure modification of this kind of compound.  
        关键词：Actinomycin D;Laser flash photolysis;DNA;BSA;Electron transfer   
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        发布时间：2023-03-30
      • ZHU Guangming,LIANG Guozheng,DI Xiyan,YU Qiuxia
        Vol. 21, Issue 2, Pages: 102-105(2003)
        摘要：The γ-radiation effects on polycaprolactones with different molecular weight were investigated. It was concluded that PCL was able to be radiation cross-linked, and the dose of gelation decreased with the increasing of molecular weight. The relation between sol fraction and the reciprocal of radiation dosage (1/D) meets Charlesby-Pinner formula. This indicates that the radiation cross-linking of PCL follows the theory of random cross-linking. Mechanical properties of radiated PCL were determined. The tensile strength and elongation at break declined with increasing doses, but the dose effects on tensile strength of high molecular weight PCL were as severe as low molecular weight PCL. Dynamic mechanical analysis showed that the heat distortion resistance and the temperature of abrupt drop point for tensile modulus E′ increased markedly with the irradiation dose.  
        关键词：Polycaprolactone;Radiation cross-linking;Charlesby-Pinner relationship   
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        发布时间：2023-03-30
      • ZHANG Junning,HONG Chengjiao,ZHU Shoupeng
        Vol. 21, Issue 2, Pages: 106-109(2003)
        摘要：The relationship between intrinsic radiosensitivity of three kinds of tumor cells, K562, LS-T-117 and C6, and the radiation-induced initial DNA single-strand breaks(SSBs) and SSB repair in these cells were investigateded by the alkaline comet assay.It was found that there was a close correlation between the initial SSBs and the delivered dose to cells: the larger the dose, the severer the SSB.For the same single dose, the different initial SSBs and intrinsic radiosensitivity of the cells measured by means of the clonogenic assay ranked as in a descending order: K562> LS-T-117>C6, while the capacity of SSB repair did as C6>LS-T-117>K562. The results suggested that both radiation-induced initial SSBs and SSB repair correlated with the intrinsic radiosensitivity of cells to a certain extent, which could be used as potential predictors for the intrinsic radiosensitivity of tumor cells.  
        关键词：Radiosensitivity for tumor cells;Alkaline comet assay;DNA single-strand breaks;DNA single-strand breaks repair   
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        发布时间：2023-03-30
      • SUN Liang,LI Shijun
        Vol. 21, Issue 2, Pages: 110-115(2003)
        摘要：In research of nuclear medicine and development of radiopharmaceuticals, it is necessary to do data analysis and know transportation behaviors of the radionuclei in tissues or organs concerned. Based on polynomial fitting method, we developed the software INDFIT 1.0 programmed by Microsoft Visual Basic 6.0(VB 6) for the data analysis. Experimental calculations showed that the software was of practical use.  
        关键词：Data;Transportation;Software;INDFIT 1.0   
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        发布时间：2023-03-30
      • LI Bingyan,TONG Jian,HONG Chengjiao,ZHANG Zengli,JIN Nianzu,ZHOU Jianwei
        Vol. 21, Issue 2, Pages: 116-119(2003)
        摘要：A study was done on DNA damage in tissue cells of ranon-inhaled rats. Sprague-Dawley rats were exposed to radon and its progeny to doses of 66, 111 and 174 WLM(working level month), respectively. Single cell suspensions of the rat lung, spleen and thymus were prepared. Single cell gel eletrophoresis(SCGE) was performed to detect the length of DNA migration in the rat peripheral blood mononuclear cells(PBMC), bronchi alveolar lavage fluids (BALF)cells, lung cells, spleen cells and thymus cells. DNA migration in the rat PBMC, BALF cells and lung cells increased with cumulative doses. The dose-effect relationships of the DNA damages and a correlation between DNA migration in PBMC and lung cells could be established. Radon and its progeny could cause DNA strand break in rat PBMC, BALF cells and lung cells.  
        关键词：Single cell gel electrophoresis (SCGE);Peripheral blood mononuclear cell (PBMC);Lung cell;DNA damage   
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        发布时间：2023-03-30
      • WU Jian,SU Mingjie,DAI Guifu,WANG Weidong,ZHANG Shu,LI Zongwei,WANG Yanping,QIN Guangyong,HUO Yuping,YUAN Tianyang,YAN Xin,GAN Chongjie
        Vol. 21, Issue 2, Pages: 120-124(2003)
        摘要：The effect of 30 keV N+ ions, UV and 60Co γ-rays on the survival and the gentamycin yield of Micromonospora purpurea were investigated. A saddle-like survival curve was found Micromonospora purpurea treated with N+ ions, whereas the curves of those irradiated by UV and 60Co γ-rays were exponential, comparisons of the induction indexes indicated that the N+ irradiation was more advantageous than UV and the γ-rays. Based on the experiments, it is concluded that induction doses of 0.5~1×1016 /cm2, i,e. the doses correlating to the peaks of the survival curve, are suitable for high yield of gentamycin.  
        关键词：Ion implantation;Micromonospora purpurea;UV;60Co γ;Nutation;Gentamycin   
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        发布时间：2023-03-30
      • QIN Quanhong,WANG Dewen,GAO Yabing,PENG Ruiyun,GU Qingyang,XIA Guowei,CUI Xuemei
        Vol. 21, Issue 2, Pages: 125-130(2003)
        摘要：The paper is to study the number, morphology and ultrastructure of fibroblasts in the process of wound healing of local irradiated rats. 156 Wistar rats were divided into two groups, the wound group (control) and the radiation-combined wound group (radiation group). Numeral, morphological and ultrastructural changes of the fibroblasts were observed with light microscope and transmission electron microscope. The α-SM actin expression in the fibroblasts was measured with immunohistochemecal assay. The number of the fibroblasts in the control group increased gradually and reached to the max on the 10th day. In the radiation group, the fibroblasts number changed less than the control and the peak was seen on the 15th day. The fibroblasts included types of the transition, the proliferation, the synthesis-secretion, the myofibroblast, the transformation and the apoptosis. For the radiation group, each of the six types of fibroblasts, especially the synthesis-secretion type and the myofibroblast type, appeared later in comparison with the control. In addition, the peculiarities of the abnormal fibroblasts could be observed in the radiation group, the highly expanded rough endoplastmic reticulums, the less golgis and microfilaments. Irradiation can inhibit the proliferation of the fibroblasts, delay the appearance of the stage-specific fibroblasts, cause the morphological and ultrastructural changes in the fibroblasts and promote the formation of the abnormal fibroblasts.  
        关键词：Irradiation;Wound healing;Fibroblast;Pathology;Ultrastructure   
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        发布时间：2023-03-30
      • MEI Kai,LIU Yongbiao,WANG Xu,HAO Xingzhi
        Vol. 21, Issue 2, Pages: 131-134(2003)
        摘要：The paper is to study radioprotective effect of Interleukin 6 (IL-6) on the spleen cells of irradiated tumor-bearing mice. After 6MV X-ray irradiation (5Gy/5f/5d), the activities of total superoxide dismutase (T-SOD) in spleens of irradiated tumor bearing mice were measured by the method of xanthineoxidase. The contents of malondialdehyde (MDA) were measured by thiobarbituric acid (TBA) fluorescence; Immunohistochemical technique was performed to detect Bcl-2 protein in spleen cells; The effects of IL-6 on natural killing activity of lymphocytes on K562 cells were determined by Beckman liquid scintillation counter IL-6 showed significant radioprotective effect on the spleen cells of irradiated tumor bearing mice, including increasing the activities of T-SOD (p<0.01) and reducing the contents of MDA (p<0.05). The increased expression level of Bcl-2 in spleen cells was obersved in the IL-6 group (p<0.01). IL-6 enhanced the natural killing activity of lymphocyte on K562 cells. Decrease of antioxidase activities and increase of free radicals may be the main causes of peroxidation induced by irradiation and damage to cell activities. IL-6 has radioprotective effect on the spleen cells, which may be due to its function of increasing the expression of Bcl-2 and antioxidase activities, eliminating free radicals, lengthening the life span of spleen cells and promoting their functions.  
        关键词：Interleukin 6;Radioprotection;Superoxide;Dismutase;MDA;Bcl-2   
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        发布时间：2023-03-30
      • GU Qingyang,WANG Dewen,ZHAO Meilan,YANG Zhixiang,GAO Yabing,YANG Hong,LIU Jie,CUI Yufang,WANG Xiaomin
        Vol. 21, Issue 2, Pages: 135-138(2003)
        摘要：To study EGF and EGFR expression during the formation and development of radiation-induced skin ulcers and their effects on ulcer healing, a rat model which was locally irradiated with 60Co γ-rays was used, and another rat model of simple skin wounds was made as a control. Pathological changes were observed for 55d. Immunohistochemistry in situ hybridization and image analysis were performed to detect EGF and EGFR expression in the tissue of radiation skin ulcers. Results: Skin ulcers were found on 14d after irradiation, and they were enlarged and deepened gradually during the observation period. In the irradiated skin, especially in the epidermal cells, fibroblasts and endothelial cells in the ulcer beds, the expression of EGF and EGFR was higher than that in normal skin. But their expression was obviously decreased in the radiation ulcer beds compared to that in the surgical wound beds. After irradiation, the decreased expression of EGF and EGFR may play an important role in the formation, development and nonhealing mechanism of radiation skin ulcers.  
        关键词：Radiation-induced skin ulcer;EGF;EGFR;Wound healing   
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        发布时间：2023-03-30
      • XU Shiqing,Kenji Kiguchi,LING Erjun,Koji Shirai,Kana Fukamoto,TU Zhenli
        Vol. 21, Issue 2, Pages: 143-147(2003)
        摘要：Eggs of silkworm, Bombyx mori were irradiated by 12C5+ ions, at the embryonic wings, where the hemopoietic organs attached. Changes in the development of the silkworm larvae and pupae, especially in the repair and regeneration of the hemopoietic organs represented by their morphological and functional characteristics, were observed. The results showed that the silkworm larvae and pupae developed from the irradiated eggs grew with the physiological obstruction due to exsanguinations, such as difficulty in their metamorphosis and their marked lower survival rates. After 100Gy irradiation, some of the larvae and pupae died during the periods of larval molting stages, pupation stage and eclosion stage. With increased doses, the deficiencies in the silkworm development became more significant. After 200Gy irradiation, the average hemocyte density for the 5th larvae decreased markedly, but the hemocyte density for some individuals were comparable to the control, and regenerated hemopoietic organs was found in these larvae. The results suggested that the silkworm larvae might be capable of self-repair and self-regeneration of the hemopoietic organs after the heave ion irradiation.  
        关键词：Heavy ion beam;Bombyx mori silkworm;Embryo;Hemopoietic organ;Repair and regeneration   
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        发布时间：2023-03-30
      • LIU Guangwei,LIU Shuchun,LU Zhe,GONG Shouliang
        Vol. 21, Issue 2, Pages: 148-153(2003)
        摘要：To study the effects of low dose radiation (LDR) with different doses of X-rays on the apoptosis in spermatogenic cells of male Kunming mouse testes. The time-effect and dose-effect of apoptosis in the different stages of spermatogenic cell cycles of mouse testis after LDR with different doses of X-rays were studied with light microscope using the methods of TdT-mediated dUTP nick end labeling (TUNEL) and HE staining. The apoptosis of spermatogenic cells induced by LDR had a remarkable regularity in cell types. When the dose was 0.025Gy, spermatogonium apoptosis was taken as main. With the dose increase of irradiation (0.025—0.2Gy), spermatocytes also showed an apoptotic change, but the apoptotic rate of spermatogonia was significantly higher than that of spermatocytes. Moreover, the apoptosis of spermatids and spermatozoa scarcely occurred after irradiation with low dose. The apoptosis of spermatogenic cells induced by LDR has a regular change, which provides a further experimental evidence for the mechanism study of hormesis by LDR.  
        关键词：Low dose radiation (LDR);Spermatogenic cells;Apoptosis;TUNEL   
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        发布时间：2023-03-30
      • CAI Weibo,ZOU Zhenghui,Qiu Jianmin,Yang Shuxian,Xu Meijuan,ZHANG Yuying,Su Liaoyuan
        Vol. 21, Issue 2, Pages: 154-156(2003)
        摘要：The paper studies hormesis of low dose radiation (LDR)in the expression of cytokines in umbilical cord blood (UCB). 1h after irradiation of UCB with 5cGy of 226Ra γ-rays, the levels of IL-2, IL-8 EPO and SOD-1 were determined by radioimmunoassay (RIA). Compared with the control (0Gy), the levels of all the assayed cytokines in the irradiated samples were up-regulated, with significant differences for IL-2, IL-8 and SOD-1 (p<0.05) and very significant difference for EPO (p<0.01). LDR with 226Ra γ-rays could induce hormesis as to the expression of IL-2, IL-8, EPO and SOD-1 in UCB.  
        关键词：Low dose radiation;Hormesis;Umbilical cord blood;Cytokines   
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        发布时间：2023-03-30
      • SUN Cuifeng,LIU Fenju,KONG Xiangrong,CHEN Jian,NING Ping
        Vol. 21, Issue 2, Pages: 157-160(2003)
        摘要：In this paper, the radioresistance of bacterium Deinococcus radiodurans to 60Co γ-rays and the relationship between the protein content in the bacterium and the irradiation dose as well as the time after irradiation were studied. By counting the colonies on the plates which grew up from the irradiated and the control bacterium, the survival rate at each irradiation dose was calculated. The protein concentration was measured with the ultraviolet spectrophotometry. The results showed that the typical survival curve of Deinococcus radiodurans had a wide shoulder up to 5kGy, characterizing the radioresistance of the bacterium. The protein concentration increased with increasing dose, reaching the maximum at 5kGy. With time prolongation after 5kGy irradiation, the protein expressed in the bacterium reduced. And the lowest protein concentration, basically the same as the control, was observed 6h after the irradiation.  
        关键词：Deinococcus radiodurans;Radioresistance;60Co γ-ray;Protein concentration   
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        发布时间：2023-03-30

        研究论文

      • YANG Zhanshan,ZHU Shoupeng,YANG Shuqin
        Vol. 21, Issue 2, Pages: 139-142(2003)
        摘要：The paper is to elucidate the effects of in vivo exposure to different levels of 235UO2F2 on DNA repair capability and the mechanism. The DNA repair capacity, as measured by UV-induced unscheduled DNA synthesis (UDS) of splenic lymphocytes from inbred BALB/c male mice, was observed after injection of different doses of 235UO2F2 into the caudal vein. UV-induced UDS of the splenic lymphocytes increased significantly (p<0.05 or p< 0.01) at doses of 0.1—20μg / kg body weight. Non-UV-induced UDS showed a significant increase (p<0.05 or p< 0.01) 12 days after 235UO2F2 injection. The UV-induced UDS in PHA activated but non-proliferating (hydroxyurea treated) lymphocytes were lower than those of lymphocytes unexposed to PHA at the same doses of 235UO2F2 (p<0.05 or p<0.01). Low doses of internally deposited 235UO2F2 have a continuous DNA damage effect on the mouse lymphocytes, so that a significant stimulative effect on DNA repair capability in the cells is induced. The stimulative effect occurs only in a limited dose range, and the dose range of internally deposited 235UO2F2 is larger than that of the external radiation. The DNA repair synthesis is significantly inhibited after UV-irradiation in PHA stimulated but non-proliferating lymphocytes.  
        关键词：235UO2F2;Lymphocytes;Unscheduled DNA synthesis;Stimulatory effect   
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        发布时间：2023-03-30
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